@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.002769, author = "Lee, Yunho and Jeon, Che Ok", title = "Siphonobacter curvatus sp. nov., isolated from a freshwater river", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2018", volume = "68", number = "6", pages = "1925-1929", doi = "https://doi.org/10.1099/ijsem.0.002769", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002769", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "taxonomy", keywords = "Bacteroidetes", keywords = "Han River", keywords = "freshwater", keywords = "Siphonobacter curvatus", abstract = "A novel orange-pigmented, Gram-stain-negative and strictly aerobic bacterium, designated strain HR-UT, was isolated from a water sample of the Han River located in the Republic of Korea. Cells were catalase-positive and oxidase-positive, and non-motile curved rods without flagella. The strain grew at 5–35 °C (optimum 25 °C), pH 6–10 (optimum of 7–8) and 0–3 % (w/v) NaCl (optimum 0 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain HR-UT formed a tight phylogenic lineage with Siphonobacter aquaeclarae DSM 21668T and ‘ Siphonobacter intestinalis’ 63MJ-2. The 16S rRNA gene sequence comparison of strain HR-UT and other reported type strains showed that it shared the highest sequence similarity to S. aquaeclarae DSM 21668T (96.9 %) and ‘S. intestinalis’ 63MJ-2 (96.6 %), and had lower similarities than 87.0 % to other bacteria with validly published names. Average nucleotide identity and in silico DNA–DNA hybridization values between strain HR-UT and the most closely related strain S. aquaeclarae were 71.7 and 18.4 %, respectively. The major respiratory quinone was menaquinone-7, and the dominant fatty acids (>5 %) consisted of C16 : 1 ω5c, summed feature 3 (comprising C16 : 1ω7c and/or iso-C15 : 0 2-OH) and iso-C15 : 0. The polar lipids comprised phosphatidylethanolamine, four unidentified aminolipids and three unidentified lipids. The DNA G+C content was 47.9 mol%. Based on the genotypic, chemotaxonomic and phenotypic analyses, strain HR-UT represents a novel species of the genus Siphonobacter , for which the name Siphonobacter curvatus sp. nov. is proposed. The type strain is HR-UT (=KACC 19409T=JCM 32267T).", }