1887

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Volume 68, Issue 6

gen. nov., sp. nov., isolated from pine forest soil and allocation of the genera and to the family fam. nov., and emendation of the genus Staley 1968 Free

Abstract

A novel bacterium, designated strain E9, was isolated from pine forest soil of Kyonggi University (Suwon, Republic of Korea). Cells were facultatively anaerobic, Gram-staining-negative, catalase-negative, oxidase-positive, non-motile, non-spore-forming, rod-shaped and straw coloured. Prosthecae were absent. Glucose was fermented. The strain grew in the pH range of 5.0–10.0 (optimum, 6.5–8.5) and at 45 °C (optimum, 28–32 °C). E9 was sensitive to NaCl at low concentration and tolerated only 0.2 % NaCl (w/v). A phylogenetic analysis based on 16S rRNA gene sequences revealed that E9 formed a lineage within the phylum that was distinct from various members of the order , including DSM 4722 (94.76 % sequence similarity), ‘’ IITR-21 (92.72 %), 16 (92.66 %) and DSM 19436 (92.53 %). The predominant isoprenoid quinone was Q-10. The major polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and phosphatidyl--methylethanolamine. Major cellular fatty acids were summed feature 8 (Cω7 and/or Cω6), summed feature 3 (Cω7 and/or Cω6), and C. The DNA G+C content of the type strain was 68.4 mol%. Polyphasic characterization indicated that strain E9 represented a novel species in a novel genus within a novel family, for which the name gen. nov., sp. nov. is proposed. The type strain of is E9 (=KEMB 9005-534=KACC 19120=NBRC 112686). A formal allocation of the genus to the family fam. nov. is also proposed.

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Keyword(s): Alphaproteobacteria , Ancalomicrobiaceae , Ancalomicrobium , facultatively anaerobic , pine forest soil and Pinisolibacter ravus
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2018-06-01
2024-04-19
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Pinisolibacter ravus gen. nov., sp. nov., isolated from pine forest soil and allocation of the genera Ancalomicrobium and Pinisolibacter to the family Ancalomicrobiaceae fam. nov., and emendation of the genus Ancalomicrobium Staley 1968
Int J Syst Evol Microbiol 68, 1955 (2018); https://doi.org/10.1099/ijsem.0.002772
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