@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.002772, author = "Dahal, Ram Hari and Chaudhary, Dhiraj Kumar and Kim, Jaisoo", title = "Pinisolibacter ravus gen. nov., sp. nov., isolated from pine forest soil and allocation of the genera Ancalomicrobium and Pinisolibacter to the family Ancalomicrobiaceae fam. nov., and emendation of the genus Ancalomicrobium Staley 1968", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2018", volume = "68", number = "6", pages = "1955-1962", doi = "https://doi.org/10.1099/ijsem.0.002772", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002772", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Alphaproteobacteria", keywords = "Ancalomicrobium", keywords = "facultatively anaerobic", keywords = "pine forest soil", keywords = "Ancalomicrobiaceae", keywords = "Pinisolibacter ravus", abstract = "A novel bacterium, designated strain E9T, was isolated from pine forest soil of Kyonggi University (Suwon, Republic of Korea). Cells were facultatively anaerobic, Gram-staining-negative, catalase-negative, oxidase-positive, non-motile, non-spore-forming, rod-shaped and straw coloured. Prosthecae were absent. Glucose was fermented. The strain grew in the pH range of 5.0–10.0 (optimum, 6.5–8.5) and at 45 °C (optimum, 28–32 °C). E9T was sensitive to NaCl at low concentration and tolerated only 0.2 % NaCl (w/v). A phylogenetic analysis based on 16S rRNA gene sequences revealed that E9T formed a lineage within the phylum Proteobacteria that was distinct from various members of the order Rhizobiales , including Ancalomicrobium adetum DSM 4722T (94.76 % sequence similarity), ‘ Nitratireductor lucknowense ’ IITR-21 (92.72 %), Prosthecomicrobium hirschii 16T (92.66 %) and Kaistia soli DSM 19436T (92.53 %). The predominant isoprenoid quinone was Q-10. The major polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and phosphatidyl-N-methylethanolamine. Major cellular fatty acids were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c), summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), and C16 : 0. The DNA G+C content of the type strain was 68.4 mol%. Polyphasic characterization indicated that strain E9T represented a novel species in a novel genus within a novel family, for which the name Pinisolibacter ravus gen. nov., sp. nov. is proposed. The type strain of Pinisolibacter ravus is E9T (=KEMB 9005-534T=KACC 19120T=NBRC 112686T). A formal allocation of the genus Ancalomicrobium to the family Ancalomicrobiaceae fam. nov. is also proposed.", }