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Volume 68, Issue 6

sp. nov., isolated from freshwater mud Free

Abstract

A Gram-stain-positive, aerobic, motile, rod- or cocci-shaped bacterium with flagella bacterium (THG-T63) was isolated from freshwater mud. Growth occurred at 10–40 °C (optimum, 28–35 °C), at pH 6–8 (optimum, 7) and at 0–6 % NaCl (optimum, 2 %). Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T63 were identified as KCTC 19470 (97.5 %), KCTC 19600 (96.4 %), KCTC 19265 (96.3 %), KCTC 19054 (96.1 %) and KCTC 457BP (96.1 %). 16S rRNA sequence similarities among strain THG-T63 and other species were lower than 96.0 %. The polar lipids were phosphatidylglycerol, phosphatidylinositol and one unidentified phospholipid. The quinone system was composed of MK-8 (H4). The major fatty acids were C, C 6, C 8, C 10-methyl, C 9 and -C. The cell-wall peptidoglycan contained -2,6-diaminopimelic acid. The DNA G+C content of strain THG-T63 was 74.6 mol%. Strain THG-T63 exhibited levels of DNA–DNA relatedness of 20–44 % to the type strains of phylogenetically related species and could be differentiated from these species based on differences in phenotypic characteristics. On the basis of the data presented here, strain THG-T63 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is THG-T63(=KACC 19192=CGMCC 4.7388).

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Keyword(s): freshwater mud , Nocardioides pelophilus and phenotypic characteristics
© 2018 IUMS
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2018-06-01
2024-04-18
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Nocardioides pelophilus sp. nov., isolated from freshwater mud
Int J Syst Evol Microbiol 68, 1942 (2018); https://doi.org/10.1099/ijsem.0.002776
/content/journal/ijsem/10.1099/ijsem.0.002776
/content/journal/ijsem/10.1099/ijsem.0.002776
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