1887

Abstract

Two bacterial strains were isolated from coal mine water in China. Isolates were facultatively anaerobic, Gram-stain-negative, rod-shaped, motile by means of a single polar flagellum, and they did not produce bacteriochlorophyll α. Cells grew in tryptic soy broth with 0–5.5 % (w/v) NaCl, at 4–55 °C and pH 3.5–10.5. Isolates were positive for catalase, oxidase, urease, Voges–Proskauer test, gelatin hydrolysis and HS production. Analysis of 16S rRNA gene sequences indicated that the closest relatives of strains Q4.6 and Q2.11 were the type strains DSM 22153 (97.4 %), DSM 14782 (96.9 and 97.0 %) and DSM 23407 (96.8 %). The genomic average nucleotide identity (ANI) value for Q4.6 and Q2.11 was 100 %; however, this value was less than 77.7 % for the type strains and , and less than 74.0 % for the type strain . The cellular fatty acid profile of strains Q4.6 and Q2.11 consisted primarily of Cω7. The principal quinone of the isolates was Q-10. The polar lipid profile consisted of diphosphatidyl glycerol, phosphatidyl glycerol, phosphatidyl ethanolamine and phosphatidyl choline. On the basis of phylogenetic analysis, genomic ANI analysis, DNA–DNA hybridization results, as well as phenotypic and chemotaxonomic data, strains Q4.6 and Q2.11 are assigned as a novel species within the genus . The type strain is Q4.6 (=CGMCC 1.15703=KCTC 52466).

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2018-06-01
2024-03-28
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