Pseudomonas tianjinensis sp. nov., isolated from domestic sewage
Chen, Peizhen and Li, Shaopeng and Li, Qing X.,, 68, 2760-2769 (2018),
doi = https://doi.org/10.1099/ijsem.0.002799,
publicationName = Microbiology Society,
issn = 1466-5026,
abstract= A novel Gram-stain-negative bacterium, designated as type strain 68T, was isolated from domestic sewage in Tianjin, China. Cells of strain 68T were aerobic, motile and rod-shaped. The organism grew at 15–42 °C, pH 6.0–11.0 and with 0–4 % NaCl (w/v). The DNA G+C content was 61.9 mol%. The major fatty acids (>10 %) were summed feature 8 (C18 : 1ω7c), summed feature 3 (C16 : 1ω7c or C16 : 1ω6c), C16 : 0 and C12 : 0. The respiratory quinone was Q9. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine and an unidentified aminophospholipid. Phylogenetic analysis based on full 16S rRNA gene sequences showed that strain 68T was a member of the genus Pseudomonas and showed similarities to Pseudomonas toyotomiensis HT-3T (98.58 %), Pseudomonas chengduensis MBRT (98.53 %), Pseudomonas alcaliphila AL15-21T (98.44 %), Pseudomonas composti C2T (97.75 %) and Pseudomonas anguilliseptica NCIMB 1949T (97.66 %). Strain 68T exhibited low DNA–DNA hybridization homology with P. alcaliphila AL15-21T (35.40 %), P. toyotomiensis HT-3T (33.30 %), P. chengduensis MBRT (35.40 %), P. anguilliseptica NCIMB 1949T (14.40 %) and P. composti C2T (20.40 %). On the basis of phenotypic, genotypic and phylogenetic evidence, strain 68T is considered to represent a novel species of the genus Pseudomonas, for which the name Pseudomonas tainjinensis sp. nov. is proposed. The type strain is 68T (=CICC 24204T=KCTC 52977T).,
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