Ketobacter alkanivorans gen. nov., sp. nov., an n-alkane-degrading bacterium isolated from seawater
Kim, Seol-Hee and Kim, Jong-Geol and Jung, Man-Young and Kim, So-Jeong and Gwak, Joo-Han and Yu, Woon-Jong and Roh, Seong Woon and Kim, Yang-Hoon and Rhee, Sung-Keun,, 68, 2258-2264 (2018),
doi = https://doi.org/10.1099/ijsem.0.002823,
publicationName = Microbiology Society,
issn = 1466-5026,
abstract= Strain GI5T was isolated from a surface seawater sample collected from Garorim Bay (West Sea, Republic of Korea). The isolated strain was aerobic, Gram-stain-negative, rod-shaped, motile by means of a polar flagellum, negative for catalase and weakly positive for oxidase. The optimum growth pH, salinity and temperature were determined to be pH 7.5–8.0, 3 % NaCl (w/v) and 25 °C, respectively; the growth ranges were pH 6.0–9.0, 1–7 % NaCl (w/v) and 18–40 °C. The results of phylogenetic analysis of 16S rRNA gene sequences indicated that GI5T clustered within the family Alcanivoracaceae , and most closely with Alcanivorax dieselolei B-5T and Alcanivorax marinus R8-12T (91.9 % and 91.6 % similarity, respectively). The major cellular fatty acids in GI5T were C18 : 1ω7c/C18 : 1ω6c (44.45 %), C16 : 1ω6c/C16 : 1ω7c (14.17 %) and C16 : 0 (10.19 %); this profile was distinct from those of the closely related species. The major respiratory quinone of GI5T was Q-8. The main polar lipids were phosphatidylethanolamine and phosphatidylglycerol. Two putative alkane hydroxylase (alkB) genes were identified in GI5T. The G+C content of the genomic DNA of GI5T was determined to be 51.2 mol%. On the basis of the results of phenotypic, chemotaxonomic and phylogenetic studies, strain GI5T represents a novel species of a novel genus of the family Alcanivoracaceae, for which we propose the name Ketobacter alkanivorans gen. nov., sp. nov.; the type strain is GI5T (=KCTC 52659T=JCM 31835T).,
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