RT Journal Article SR Electronic(1) A1 Kämpfer, Peter A1 Glaeser, Stefanie P. A1 Packroff, Gabriele A1 Behringer, Katja A1 Exner, Martin A1 Chakraborty, Trinad A1 Schmithausen, Ricarda M. A1 Doijad, SwapnilYR 2018 T1 Lelliottia aquatilis sp. nov., isolated from drinking water JF International Journal of Systematic and Evolutionary Microbiology, VO 68 IS 8 SP 2454 OP 2461 DO https://doi.org/10.1099/ijsem.0.002854 PB Microbiology Society, SN 1466-5034, AB Five beige-pigmented, oxidase-negative bacterial isolates, 6331-17T, 6332-17, 6333-17, 6334-17 and 9827-07, isolated either from a drinking water storage reservoir or drinking water in 2006 and 2017 in Germany, were examined in detail applying by a polyphasic taxonomic approach. Cells of the isolates were rod-shaped and Gram-stain-negative. Comparison of the 16S rRNA gene sequences of these five isolates showed highest sequence similarities to Lelliottia amnigena (99.98 %) and Lelliottia nimipressuralis (99.99 %). Multilocus sequence analyses based on concatenated partial rpoB, gyrB, infB and atpD sequences confirmed the clustering of these isolates with Lelliottia species, but also revealed a clear distinction to the closest related type strains. Analysis of the genome sequences of these isolates indicated >70 % in silico DNA–DNA hybridization and high average nucleotide identities between strains. Nevertheless, they showed only <70 and <95 % similarity to the type strains of these two Lelliottia species. The fatty acid profiles of these isolates were very similar and consisted of the major fatty acids C16:0, C17 : 0cyclo, C15 : 0iso 2-OH/C16 : 1ω7c and C18 : 1 ω7c. In addition, physiological/biochemical tests revealed high phenotypic similarity to each other. These cumulative data indicate that these isolates represent a novel Lelliottia species, for which the name Lelliottia aquatilis sp. nov. is proposed, with strain 6331-17T (=CCM 8846T=CIP 111609T=LMG 30560T) as the type strain., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002854