@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.002864, author = "Cao, Chengliang and Sun, Yong and Wu, Bo and Zhao, Shuai and Yuan, Bo and Qin, Sheng and Jiang, Jihong and Huang, Ying", title = "Actinophytocola glycyrrhizae sp. nov. isolated from the rhizosphere of Glycyrrhiza inflata", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2018", volume = "68", number = "8", pages = "2504-2508", doi = "https://doi.org/10.1099/ijsem.0.002864", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002864", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "rhizospheric soil", keywords = "16S rRNA gene", keywords = "polyphasic taxonomy", keywords = "Pseudonocardiaceae", abstract = "A Gram-stain-positive, aerobic actinomycete, designated strain BMP B8152T, was isolated from the rhizosphere of Glycyrrhiza inflata collected ashore, in Kashi, Xinjiang province, northwest PR China. A polyphasic approach was used to establish the taxonomic position of this strain. BMP B8152T was observed to form non-fragmented substrate mycelium, and relatively scanty aerial mycelium with rod-shaped spores. Cell-wall hydrolysates contained meso-diaminopimelic acid, galactose, arabinose, glucose and rhamnose (trace). Mycolic acids were not detected. The diagnostic phospholipids were identified as diphosphatidylglycerol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine, ninhydrin-positive phosphoglycolipid and phosphatidylinositol. The predominant menaquinone and fatty acid were MK-9(H4) and iso-branched hexadecanoate (iso-C16 : 0), respectively. The phylogenetic analyses based on the 16S rRNA gene sequences indicated that BMP B8152T formed a distinct monophyletic clade clustered with Actinophytocola timorensis ID05-A0653T (98.8 % 16S rRNA gene sequence similarity), Actinophytocola oryzae GMKU 367T (98.6 %), Actinophytocola corallina ID06-A0464T (98.2 %) and Actinophytocola burenkhanensis MN08-A0203T (97.5 %). In addition, DNA–DNA hybridization values between BMP B8152T and A. timorensis ID05-A0653T(44.2±3.6 %) and A. oryzae GMKU 367T(36.7±2.3 %) were well below the 70 % limit for species identification. The combined phenotypic and genotypic data indicate that the isolate represents a novel species of the genus Actinophytocola , for which the name Actinophytocola glycyrrhizae sp. nov., is proposed, with the type strain BMP B8152T (=KCTC 49002T=CGMCC 4.7433T).", }