RT Journal Article SR Electronic(1) A1 Zhou, Xing-Kui A1 Mi, Qi-Li A1 Yao, Jian-Hua A1 Wu, Heng A1 Liu, Xiu-Ming A1 Li, Yuan-Dong A1 Duan, Yan-Qing A1 Chen, Jian-Hua A1 Dang, Li-Zhi A1 Mo, Ming-He A1 Li, Xue-Mei A1 Li, Wen-JunYR 2018 T1 Sphingomonas tabacisoli sp. nov., a member of the genus Sphingomonas, isolated from rhizosphere soil of Nicotiana tabacum L. JF International Journal of Systematic and Evolutionary Microbiology, VO 68 IS 8 SP 2574 OP 2579 DO https://doi.org/10.1099/ijsem.0.002879 PB Microbiology Society, SN 1466-5034, AB A Gram-staining-negative, aerobic, motile and rod-shaped bacterium, designated strain X1-8T, was isolated from rhizosphere soil of Nicotiana tabacum L. collected from the tobacco produce base located in Kunming, south-west PR China. Cells showed oxidase-negative and catalase-positive reactions and were motile by means of peritrichous flagella. Growth occurred at 25–40 °C and pH 6.0–8.0 with optimal growth at 30–35 °C, pH 7.0. The major respiratory lipoquinone was Q-10. C16 : 0 and summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) were identified as major cellular fatty acids. The profile of polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, sphingoglycolipid, phosphatidylcholine and one unidentified glycolipid. The major polyamine was sym-homospermidine. The genomic DNA G+C content was 66.5 mol%. The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that X1-8T should be affiliated to the genus Sphingomonas and formed a clade with most closely related species Sphingomonas changbaiensis NBRC 104936T. The results of 16S rRNA gene sequences similarity analysis indicated that X1-8T had the highest similarity with S. changbaiensis NBRC 104936T (98.4 %) and lower than 96.0 % with other species of the genus Sphingomonas . DNA–DNA hybridization data indicated that X1-8T represented a novel genomic species of the genus Sphingomonas . The characteristics determined in the polyphasic taxonomic study indicated that X1-8T represents a novel species of the genus Sphingomonas , for which the name Sphingomonas tabacisoli sp. nov. (type strain X1-8T=KCTC 62032T=CGMCC 1.16275T) is proposed., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002879