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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 68, Issue 9

Clostridium beihaiense sp. nov., an anaerobic bacterium isolated from activated sludge Free

Abstract

A Gram-positive, strictly anaerobic, rod-shaped bacterium, designated YB-7, was isolated from activated sludge of an anaerobic baffled reactor pond in Weizhou terminal wastewater treatment plant, Beihai, Guangxi, China. Strain YB-7 grew at pH 5.0–12.0 (optimum, pH 7.0), 20–45 °C (37 °C) and NaCl concentration of 0–5 % w/v (optimum, 5 %). 16S rRNA gene sequence analysis results showed that strain YB-7 belonged to the genus Clostridium and it was most closely related to Clostridium tetanomorphum DSM 4474 (96.9 % similarity). The DNA–DNA relatedness of strain YB-7 to Clostridium tetanomorphum DSM 4474 was 47.4 %. The DNA G+C content of strain YB-7 was determined to be 32.3 mol%, and the predominant cellar fatty acid (>10 %) was C16 : 0. Polar lipids of strain YB-7 included diphosphatidylglycerol, phosphatidylethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, two unidentified aminophospholipids, two unidentified phospholipids and unidentified lipids. The results of this study supported the conclusion that strain YB-7 should be assigned to a new member of the genus Clostridium , for which the name Clostridium beihaiense sp. nov. is proposed. The type strain is YB-7 (=CICC 24109=KCTC 15555).

  • Received:
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Keyword(s): anaerobe , Clostridium beihaiense sp. nov. , new species and taxonomy
© 2018 IUMS
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2018-07-20
2024-04-19
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Clostridium beihaiense sp. nov., an anaerobic bacterium isolated from activated sludge
Int J Syst Evol Microbiol 68, 2789 (2018); https://doi.org/10.1099/ijsem.0.002885
/content/journal/ijsem/10.1099/ijsem.0.002885
/content/journal/ijsem/10.1099/ijsem.0.002885
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