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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 68, Issue 9

Saccharospirillum mangrovi sp. nov., a bacterium isolated from mangrove sediment Free

Abstract

A Gram-negative, spirilla, non-spore-forming, motile and strictly aerobic bacterium, designated HK-33, was isolated from a mangrove sediment sample in Haikou city, Hainan Province, China. Strain HK-33 was able to grow at 10–45 °C (optimum 37 °C), 0.5–12.0 % (w/v) NaCl (2.0 %, w/v) and pH 5.5–8.5 (pH 7.0). The major cellular fatty acids were C16 : 0 and summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c). Ubiquinone-8 was the predominant respiratory quinone, and Q-9 was present in trace amounts. The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two unidentified phospholipids, two unidentified glycolipids, an unidentified aminophosphoglycolipid and two unidentified lipids. The DNA G+C content was 57.3 mol%. According to 16S rRNA gene sequences similarity, strain HK-33 shared 98.6 %, 96.4%, 95.7 and 94.9 % sequence similarities to the species Saccharospirillum correiae CPA1, Saccharospirillum impatiens EL-105, Saccharospirillum salsuginis YIM-Y25 and Saccharospirillum aestuarii IMCC 4453, respectively. Phylogenetic analysis showed that strain HK-33 was clustered with S. correiae CPA1, S. impatiens EL-105, S. salsuginis YIM-Y25 and S. aestuarii IMCC 4453. Results of DNA–DNA hybridization analysis revealed that strain HK-33 shared 36.3±1.7 % DNA relatedness with S. correiae CPA1. On the basis of its phenotypic, chemotaxonomic and genotypic characteristics, strain HK-33 is considered to represent a novel species in the genus Saccharospirillum , for which the name Saccharospirillum mangrovi sp. nov. is proposed. The type strain is HK-33 (=KCTC 62178=MCCC 1K03440).

  • Received:
  • Accepted:
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Keyword(s): mangrove sediment , Saccharospirillum mangrovi and taxonomy
© 2018 IUMS
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2018-07-17
2024-04-25
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Saccharospirillum mangrovi sp. nov., a bacterium isolated from mangrove sediment
Int J Syst Evol Microbiol 68, 2813 (2018); https://doi.org/10.1099/ijsem.0.002899
/content/journal/ijsem/10.1099/ijsem.0.002899
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