RT Journal Article SR Electronic(1) A1 Zhang, Weiyan A1 Yuan, Ye A1 Su, Dengquan A1 Ding, Lijian A1 Yan, Xiaojun A1 Wu, Min A1 Epstein, Slava S. A1 He, ShanYR 2018 T1 Saccharospirillum mangrovi sp. nov., a bacterium isolated from mangrove sediment JF International Journal of Systematic and Evolutionary Microbiology, VO 68 IS 9 SP 2813 OP 2818 DO https://doi.org/10.1099/ijsem.0.002899 PB Microbiology Society, SN 1466-5034, AB A Gram-negative, spirilla, non-spore-forming, motile and strictly aerobic bacterium, designated HK-33T, was isolated from a mangrove sediment sample in Haikou city, Hainan Province, China. Strain HK-33T was able to grow at 10–45 °C (optimum 37 °C), 0.5–12.0 % (w/v) NaCl (2.0 %, w/v) and pH 5.5–8.5 (pH 7.0). The major cellular fatty acids were C16 : 0 and summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c). Ubiquinone-8 was the predominant respiratory quinone, and Q-9 was present in trace amounts. The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two unidentified phospholipids, two unidentified glycolipids, an unidentified aminophosphoglycolipid and two unidentified lipids. The DNA G+C content was 57.3 mol%. According to 16S rRNA gene sequences similarity, strain HK-33T shared 98.6 %, 96.4%, 95.7 and 94.9 % sequence similarities to the species Saccharospirillum correiae CPA1T, Saccharospirillum impatiens EL-105T, Saccharospirillum salsuginis YIM-Y25T and Saccharospirillum aestuarii IMCC 4453T, respectively. Phylogenetic analysis showed that strain HK-33T was clustered with S. correiae CPA1T, S. impatiens EL-105T, S. salsuginis YIM-Y25T and S. aestuarii IMCC 4453T. Results of DNA–DNA hybridization analysis revealed that strain HK-33T shared 36.3±1.7 % DNA relatedness with S. correiae CPA1T. On the basis of its phenotypic, chemotaxonomic and genotypic characteristics, strain HK-33T is considered to represent a novel species in the genus Saccharospirillum , for which the name Saccharospirillum mangrovi sp. nov. is proposed. The type strain is HK-33T (=KCTC 62178T=MCCC 1K03440T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002899