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Volume 68, Issue 9

Euzebya rosea sp. nov., a rare actinobacterium isolated from the East China Sea and analysis of two genome sequences in the genus Euzebya Free

Abstract

Rare Actinobacteria , known as non- Streptomyces , hold great potential to produce new bioactive compounds for drug development. A strain designated DSW09, which belongs those rare Actinobacteria , was isolated from surface seawater of the East China Sea. The cells were aerobic, Gram-positive, non-motile, non-spore-forming and rod-shaped (0.4 µm wide and 1.5–4.0 µm long). The closest relative was Euzebya tangerina F10 (96.46 % of 16S rRNA gene similarity). Cell growth occurred at 15–45 °C (optimum, 25–30 °C), at pH 6.0–9.0 (pH 6.0–7.0) and at NaCl concentrations of 0.5–5.0 % (w/v; 1.0–4.0 %). The major cellular fatty acids were summed feature 3 (comprising C16 : 1 ω7c and/or C15 : 0 iso 2OH), C17 : 1 ω8c and C16 : 0. The predominant polar lipid was diphosphatidylglycerol. The predominant menaquinone was MK-9(H4). The cell-wall peptidoglycan was A1 γ–type, containing meso-DPA. The major cell-wall sugars were rhamnose and ribose. The genome size was 5 509 297 bp with a 71.29 mol% G+C content for strain DSW09, while 4 781 440 bp with a 68.87 mol% G+C content for E. tangerina F10. The average nucleotide identity and digital DNA–DNA hybridization values between strain DSW09 and E. tangerina F10 were 73.44 % and 16.43 %, respectively. Based on phylogenetic, phenotypic, chemotaxonomic evidence and genomic analyses, strain DSW09 is a novel species of genus Euzebya , for which the name Euzebya rosea sp. nov. is proposed. The type strain is DSW09 (=DMS 104446=MCCC 1K03290).

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Keyword(s): Euzebya rosea , rare Actinobacteria , surface seawater , taxonomy and whole genome sequence
© 2018 IUMS
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2018-07-16
2024-04-19
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Euzebya rosea sp. nov., a rare actinobacterium isolated from the East China Sea and analysis of two genome sequences in the genus Euzebya
Int J Syst Evol Microbiol 68, 2900 (2018); https://doi.org/10.1099/ijsem.0.002917
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