RT Journal Article SR Electronic(1) A1 Yun, Bo-Ram A1 Park, Sunjoo A1 Kim, Min-Kyeong A1 Park, Jisun A1 Kim, Seung BumYR 2018 T1 Shewanella saliphila sp. nov., Shewanella ulleungensis sp. nov. and Shewanella litoralis sp. nov., isolated from coastal seawater JF International Journal of Systematic and Evolutionary Microbiology, VO 68 IS 9 SP 2960 OP 2966 DO https://doi.org/10.1099/ijsem.0.002929 PB Microbiology Society, SN 1466-5034, AB Three Gram-negative, non-spore-forming, rod-shaped and motile bacterial strains, designated MMS16-UL250T, MMS16-UL253T and MMS16-UL482T, were isolated from coastal seawater and subjected to taxonomic characterization. All isolates grew at 4–30 °C (optimum, 25 °C), at pH 6–10 (pH 7) and in the presence of up to 8 % NaCl (2.5–4.5 %). The 16S rRNA gene sequence similarities between the three isolates and Shewanella algicola St-6T, the closest species, were 98.1–99.2 %, and those among the isolates were 98.5–99.0 %. In the phylogenetic tree, MMS16-UL250T formed a cluster with S. algicola St-6T, but the DNA–DNA relatedness between the two strains was 28.8±1.5 %, thus confirming their separation at species level. The other two strains formed separate phylogenetic lines respectively. The main quinones for all strains were Q-7, Q-8, MK-7 and MMK-7, which is typical for Shewanella . The major polar lipids of all strains were phosphatidylglycerol and phosphatidylethanolamine, and the common major fatty acid was a summed feature consisting of C16 : 1ω7c and/or C16 : 1ω6c while the proportions varied among the three strains. The DNA G+C contents of the strains also varied between 42.1 and 43.7 mol%. Phenotypic properties distinguished each strain from S. algicola as well as from one another. Based on the polyphasic analysis, each strain is considered to represent a novel species of Shewanella , for which the names Shewanella saliphila sp. nov. (type strain, MMS16-UL250T=KCTC 62131T=JCM 32304T), Shewanella ulleungensis sp. nov. (type strain, MMS16-UL253T=KCTC 62130T=JCM 32305T) and Shewanella litoralis sp. nov. (type strain, MMS16-UL482T=KCTC 62129T=JCM 32306T) are proposed., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002929