Lactobacillus porci sp. nov., isolated from small intestine of a swine
Kim, Ji-Sun and Choe, Hanna and Kim, Kyung Mo and Lee, Yu-Ri and Rhee, Moon-Soo and Park, Doo-Sang,, 68, 3118-3124 (2018),
doi = https://doi.org/10.1099/ijsem.0.002949,
publicationName = Microbiology Society,
issn = 1466-5026,
abstract= A facultatively anaerobic, Gram-stain-positive, catalase-negative, rod-shaped lactic acid bacterium, designated SG816T, was isolated from small intestine of a swine. Optimal growth occurred at 37 °C and pH 7.0. Furthermore, growth occurred in NaCl up to 0.5 % (w/v) but not at levels of salinity higher than 1 %. Comparative 16S rRNA gene sequencing and the matrix-associated laser desorption/ionization–time-of-flight mass spectometry profiling showed that strain SG816T was closely related to Lactobacillus delbrueckii subsp. bulgaricus KCTC 3635T (95.9 %) and Lactobacillus delbrueckii subsp. indicus JCM 15610T (95.9 %), followed by other Lactobacillus delbrueckii subspecies (95.9–95.7 %) and Lactobacillus equicursoris DSM 19284T (95.6 %). A comparison of two housekeeping genes, RNA polymerase alpha subunit (rpoA) and phenylalanyl-tRNA synthase alpha subunit (pheS), revealed that strain SG816T formed a separate branch within the clade of the genus Lactobacillus . The DNA G+C content level of the strain SG816T was 51.5 mol%. The strain was homofermentative and produced d-lactic acid from glucose fermentation. The major cellular fatty acids (>10 %) of the isolate were C18 : 1ω9c and C16 : 0. The peptidoglycan type was A4α l-Lys-d-Asp. On the basis of distinct phenotypic and phylogenetic properties, strain SG816T represents a novel species of the genus Lactobacillus , for which the name Lactobacillus porci sp. nov. is proposed. The type strain is SG816T (=KCTC 21090T=NBRC 112917T).,
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