RT Journal Article SR Electronic(1) A1 Cho, Eui-Sang A1 Cha, In-Tae A1 Roh, Seong Woon A1 Nam, Young-Do A1 Seo, Myung-JiYR 2018 T1 Haloplanus rallus sp. nov., a halophilic archaeon isolated from crude solar salt JF International Journal of Systematic and Evolutionary Microbiology, VO 68 IS 10 SP 3226 OP 3231 DO https://doi.org/10.1099/ijsem.0.002970 PB Microbiology Society, SN 1466-5034, AB A halophilic archaeon, strain MBLA0036T, was isolated from Sorae solar saltern near Incheon, Republic of Korea. Strain MBLA0036T had three 16S rRNA genes: rrnA, rrnB and rrnC. The 16S rRNA gene sequence similarities between strain MBLA0036T (based on the rrnA gene) and Haloplanus ruber R35T and Haloplanus litoreus GX21T were 98.0 and 97.3 %, respectively. The similarities of the RNA polymerase subunit B′ gene between strain MBLA0036T and H. ruber R35T and H. litoreus GX21T were 94.0 and 92.1 %, respectively. Cells of strain MBLA0036T were Gram-stain-negative, motile, red-pigmented, pleomorphic, flat and contained gas vesicles. Strain MBLA0036T grew at 15‒55 °C (optimum, 37 °C), in 10‒30 % (w/v) NaCl (15 %, w/v) with 0‒0.5 M MgSO4.7H2O (0.2 M) and at pH 6.0–9.0 (pH 7.0). The cells lysed in distilled water and the minimum NaCl concentration that prevented cell lysis was 5 % (w/v). Major polar lipids of strain MBLA0036T were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and a glycolipid that was chromatographically identical to sulfated mannosyl glucosyl diether. The major isoprenoid quinone was menaquinone-8. The genomic DNA G+C content was 65.5 mol%. DNA–DNA hybridization values between strain MBLA0036T and H. ruber JCM 17271T and H. litoreus JCM 17092T were 35±3 and 18±1 %, respectively. Therefore, strain MBLA0036T is described a novel species of the Haloplanus , for which we propose the name Haloplanus rallus sp. nov. The type strain is MBLA0036T (=KCTC 4239T=JCM 31425T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002970