1887

Abstract

An endophytic strain (designated as strain SYPF 8335) was isolated from a root of Panax notoginseng in Wenshan district, Yunnan province of China. Strain SYPF 8335 grew very slowly and formed white colonies. Phylogenetic analysis of four loci indicated that strain SYPF 8335 was placed in the Drechmeria clade with Drechmeria campanulata as its closest phylogenetic neighbour. The nucleotide differences between strain SYPF 8335 and D. campanulata are 30 substitutions in the internal transcriber region region. A key morphological feature that differentiates the two fungi is that D. campanulata produces campanulate conidia. Combined with the morphology and molecular analyses, a new species named Drechmeria panacis sp. nov., is proposed.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.002971
2018-08-16
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/68/10/3255.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.002971&mimeType=html&fmt=ahah

References

  1. Gams W, Jansson HB. The nematode parasite Meria coniospora Drechsler in pure culture and its classification. Mycotaxon 1985; 22:33–38
    [Google Scholar]
  2. Sung GH, Hywel-Jones NL, Sung JM, Luangsa-Ard JJ, Shrestha B et al. Phylogenetic classification of Cordyceps and the clavicipitaceous fungi. Stud Mycol 2007; 57:5–59 [View Article][PubMed]
    [Google Scholar]
  3. Zare R, Gams W. A revision of Verticillium sect. Prostrata. VI. The genus Haptocillium. Nova Hedwigia 2001; 73:271–292
    [Google Scholar]
  4. Glockling SL, Dick MW. Two new species of nematophagous Verticillium (Hyphomycetes) forming catenulate conidia, with a key to the nematophagous Verticillium species. Nord J Bot 1997; 17:653–662 [View Article]
    [Google Scholar]
  5. Zhang K-Q, Cao L, Liang Z-Q. A new nematophagous species of Verticillium from China. Mycol Res 1996; 100:1481–1482 [View Article]
    [Google Scholar]
  6. Goodey JB. A new species of hyphomycete attacking the stem eelworm Ditylenchus dipsaci. Trans Br Mycol Soc 1951; 34:270–272 [View Article]
    [Google Scholar]
  7. Dowsett JA, Reid J, Hopkin A. On Cephalosporium balanoides Drechsler. Mycologia 1982; 74:687–690 [View Article]
    [Google Scholar]
  8. Gams W, Zare R. A revision of Verticillium sect. Prostrata. III. Generic classification. Nova Hedwigia 2001; 72:329–337
    [Google Scholar]
  9. Glockling SL, Holbrook GP. Endoparasites of soil nematodes and rotifers II the genus Haptocillium. Mycologist 2005; 19:2–9 [View Article]
    [Google Scholar]
  10. Quandt CA, Kepler RM, Gams W, Araújo JP, Ban S et al. Phylogenetic-based nomenclatural proposals for Ophiocordycipitaceae (Hypocreales) with new combinations in Tolypocladium. IMA Fungus 2014; 5:121–134 [View Article][PubMed]
    [Google Scholar]
  11. Spatafora JW, Quandt CA, Kepler RM, Sung GH, Shrestha B et al. New 1F1N species combinations in Ophiocordycipitaceae (Hypocreales). IMA Fungus 2015; 6:357–362 [View Article][PubMed]
    [Google Scholar]
  12. Dingley JM. The Hypocreales of New Zealand V. The genera Cordyceps and Torrubiella. Trans Roy Soc NZ 1953; 81:329–343
    [Google Scholar]
  13. Choi RC, Zhu JT, Leung KW, Chu GK, Xie HQ et al. A flavonol glycoside, isolated from roots of Panax notoginseng, reduces amyloid-beta-induced neurotoxicity in cultured neurons: signaling transduction and drug development for Alzheimer's disease. J Alzheimers Dis 2010; 19:795–811 [View Article][PubMed]
    [Google Scholar]
  14. Cicero AF, Vitale G, Savino G, Arletti R. Panax notoginseng (Burk.) effects on fibrinogen and lipid plasma level in rats fed on a high-fat diet. Phytother Res 2003; 17:174–178 [View Article][PubMed]
    [Google Scholar]
  15. Wang CZ, Luo X, Zhang B, Song WX, Ni M et al. Notoginseng enhances anti-cancer effect of 5-fluorouracil on human colorectal cancer cells. Cancer Chemother Pharmacol 2007; 60:69–79 [View Article][PubMed]
    [Google Scholar]
  16. Zheng YK, Miao CP, Chen HH, Huang FF, Xia YM et al. Endophytic fungi harbored in Panax notoginseng: diversity and potential as biological control agents against host plant pathogens of root-rot disease. J Ginseng Res 2017; 41:353–360 [View Article][PubMed]
    [Google Scholar]
  17. Tan Y, Cui Y, Li H, Kuang A, Li X et al. Rhizospheric soil and root endogenous fungal diversity and composition in response to continuous Panax notoginseng cropping practices. Microbiol Res 2017; 194:10–19 [View Article][PubMed]
    [Google Scholar]
  18. Jin Z, Gao L, Zhang L, Liu T, Yu F et al. Antimicrobial activity of saponins produced by two novel endophytic fungi from Panax notoginseng. Nat Prod Res 2017; 31:2700–2703 [View Article][PubMed]
    [Google Scholar]
  19. Liu K, Yang YB, Chen JL, Miao CP, Wang Q et al. Koninginins N-Q, Polyketides from the endophytic fungus Trichoderma koningiopsis harbored in Panax notoginseng. Nat Prod Bioprospect 2016; 6:49–55 [View Article][PubMed]
    [Google Scholar]
  20. Xie J, Wu YY, Zhang TY, Zhang MY, Zhu WW et al. New and bioactive natural products from an endophyte of Panax notoginseng. RSC Adv 2017; 7:38100–38109 [View Article]
    [Google Scholar]
  21. Qin S, Li J, Chen HH, Zhao GZ, Zhu WY et al. Isolation, diversity, and antimicrobial activity of rare actinobacteria from medicinal plants of tropical rain forests in Xishuangbanna, China. Appl Environ Microbiol 2009; 75:6176–6186 [View Article][PubMed]
    [Google Scholar]
  22. Qin S, Xing K, Jiang JH, Xu LH, Li WJ. Biodiversity, bioactive natural products and biotechnological potential of plant-associated endophytic actinobacteria. Appl Microbiol Biotechnol 2011; 89:457–473 [View Article][PubMed]
    [Google Scholar]
  23. Zhang T, Deng X, Yu Y, Zhang M, Zhang Y. Pseudochaetosphaeronema ginkgonis sp. nov., an endophyte isolated from Ginkgo biloba. Int J Syst Evol Microbiol 2016; 66:4377–4381 [View Article][PubMed]
    [Google Scholar]
  24. White TJ, Bruns T, Lee S, Taylor J. PCR protocols: a guide to methods and applications. In Amplification and Direct Sequencing of Fungal Ribosomal Rna Genes for Phylogenetics San Diego, CA: Academic Press; 1990 pp. 315–322
    [Google Scholar]
  25. Rehner SA, Samuels GJ. Molecular systematics of the Hypocreales: a teleomorph gene phylogeny and the status of their anamorphs. Can J Bot 1995; 73:816–823 [View Article]
    [Google Scholar]
  26. Vilgalys R, Hester M. Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. J Bacteriol 1990; 172:4238–4246 [View Article][PubMed]
    [Google Scholar]
  27. Rehner SA, Buckley E. A Beauveria phylogeny inferred from nuclear ITS and EF1-alpha sequences: evidence for cryptic diversification and links to Cordyceps teleomorphs. Mycologia 2005; 97:84–98[PubMed]
    [Google Scholar]
  28. Jaklitsch WM, Komon M, Kubicek CP, Druzhinina IS. Hypocrea voglmayrii sp. nov. from the Austrian Alps represents a new phylogenetic clade in Hypocrea/Trichoderma. Mycologia 2005; 97:1365–1378 [View Article][PubMed]
    [Google Scholar]
  29. Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA et al. Clustal W and Clustal X version 2.0. Bioinformatics 2007; 23:2947–2948 [View Article][PubMed]
    [Google Scholar]
  30. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33:1870–1874 [View Article][PubMed]
    [Google Scholar]
  31. Stamatakis A. RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 2014; 30:1312–1313 [View Article][PubMed]
    [Google Scholar]
  32. Ronquist F, Teslenko M, van der Mark P, Ayres DL, Darling A et al. MrBayes 3.2: efficient bayesian phylogenetic inference and model choice across a large model space. Syst Biol 2012; 61:539–542 [View Article][PubMed]
    [Google Scholar]
  33. Gazis R, Skaltsas D, Chaverri P. Novel endophytic lineages of Tolypocladium provide new insights into the ecology and evolution of Cordyceps-like fungi. Mycologia 2014; 106:1090–1105 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.002971
Loading
/content/journal/ijsem/10.1099/ijsem.0.002971
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error