Lactobacilus nuruki sp. nov., isolated from Nuruk, a Korean fermentation starter
Heo, Jun and Saitou, Satomi and Tamura, Tomohiko and Cho, Hayoung and Kim, Ji-Seon and Joa, Jae-Ho and Kim, Jeong-Seon and Kwon, Soon-Wo and Kim, Soo-Jin,, 68, 3273-3278 (2018),
doi = https://doi.org/10.1099/ijsem.0.002976,
publicationName = Microbiology Society,
issn = 1466-5026,
abstract= A rod-shaped, Gram-stain-positive, non-flagellated, facultatively anaerobic bacterium, designated SYF10-1aT, was isolated from Nuruk, a Korean traditional fermentation starter. It grew at 4–40 °C (optimum 37 °C), at pH 3.0–9.0 (optimum, pH 7.0) and with 0–5 % (w/v) NaCl. Phylogenetic analysis using 16S rRNA gene sequences revealed that strain SYF10-1aT belonged to the genus Lactobacillus and showed the highest sequence similarity of 98.7 % to Lactobacillus crustorum LMG 23699T. A comparison of two housekeeping genes, pheS and rpoA, supported the suggestion that strain SYF10-1aT fell within the radius of the genus Lactobacillus , but was clearly separated from its closest related species. The average nucleotide identity value and digital DNA–DNA hybridization value between strain SYF10-1aT and the most closely related species, L. crustorum LMG 23699T, were 80.5 and 33.3 %, respectively. The predominant cellular fatty acids were C16 : 0, C18 : 1ω9c, C18 : 1ω7c and summed feature 3 (including iso-C15 : 0 2-OH and/or C16 : 1ω7c). Polar lipids were diphosphatidylglycerol, phosphatidylglycerol, two unidentified aminolipids and two unidentified glycolipids. The cell-wall peptidoglycan was of the A4α type with an interpeptide bridge comprising l-Lys-d-Asp. The DNA G+C content was 34.2 mol%. On the basis of this taxonomic study, strain SYF10-1aT represents a novel species within the genus Lactobacillus , for which the name Lactobacillus nuruki sp. nov. is proposed. The type strain is SYF10-1aT (=KACC 18726T=NBRC 112011T).,
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