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Chitinophaga parva sp. nov., a new member of the family Chitinophagaceae, isolated from soil in a chemical factory

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Chitinophaga parva sp. nov., a new member of the family Chitinophagaceae, isolated from soil in a chemical factory, Page 1 of 1

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  • A Gram-stain-negative bacterium, designated LY-1, was isolated from the soil sample collected from a chemical factory in Fuyang city, Anhui province, China. Cells of strain LY-1 were strictly aerobic, non-motile and rod-shaped. Strain LY-1 grew optimally at pH 7.0 and at 30–35 °C. The taxonomic position was investigated using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain LY-1 was a member of the genus Chitinophaga and showed the highest sequence similarity to Chitinophaga costaii A37T2 (97.5 %) and lower (<97.0 %) sequence similarity to other known Chitinophaga species. Chemotaxonomic analysis revealed that strain LY-1 possessed menaquinone-7 as the major isoprenoid quinone; and iso-C15 : 0 (46.4 %), C16 : 1ω5c (27.8 %) and iso-C17 : 0 3-OH (9.0 %) were the predominant fatty acids. The polar lipids of strain LY-1 consisted of phosphatidylethanolamine, three unidentified phosphoaminolipids, one unidentified phospholipid, four unidentified lipids, two unidentified aminolipids and two unidentified glycolipids. The genomic DNA G+C content of strain LY-1 was 52.4 mol% based on total genome calculations. The average nucleotide identity and the digital DNA–DNA hybridization value of the draft genomes between strain LY-1 and strain A37T2 were 76.8 and 19.8 %, respectively. Based on the phylogenetic and phenotypic characteristics, chemotaxonomic data, and DNA–DNA hybridization, strain LY-1 is considered a novel species of the genus Chitinophaga , for which the name Chitinophaga parva sp. nov. (type strain LY-1=CCTCC AB 2018018=KCTC 62444) is proposed.

  • The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of Chitinophaga parva LY-1 is MG871256. The GenBank/EMBL/DDBJ accession numbers for the draft genome sequences of Chitinophaga parva LY-1 and Chitinophaga costaii A37T2 are QCYK00000000 and QCYL00000000, respectively.

  • Four supplementary figures are available with the online version of this article.

© 2018 IUMS | Published by the Microbiology Society

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/content/journal/ijsem/10.1099/ijsem.0.003006
2018-09-12
2019-02-19
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