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Volume 68, Issue 11

Romboutsia hominis sp. nov., the first human gut-derived representative of the genus Romboutsia, isolated from ileostoma effluent Free

Abstract

A Gram-stain-positive, motile, rod-shaped, obligately anaerobic bacterium, designated FRIFI, was isolated from human ileostoma effluent and characterized. On the basis of 16S rRNA gene sequence similarity, strain FRIFI was most closely related to the species Romboutsia ilealis CRIB (97.7 %), Romboutsia lituseburensis DSM 797 (97.6 %) and Romboutsia sedimentorum LAM201 (96.6 %). The level of DNA–DNA relatedness between strain FRIFI and R. ilealis CRIB was 13.9±3.3 % based on DNA–DNA hybridization. Whole genome sequence-based average nucleotide identity between strain FRIFI and closely related Romboutsia strains ranged from 78.4–79.1 %. The genomic DNA G+C content of strain FRIFI was 27.8 mol%. The major cellular fatty acids of strain FRIFI were saturated and unsaturated straight-chain C12–C19 fatty acids as well as cyclopropane fatty acids, with C16 : 0 being the predominant fatty acid. The polar lipid profile comprised five phospholipids and six glycolipids. These results, together with differences in phenotypic features, support the proposal that strain FRIFI represents a novel species within the genus Romboutsia , for which the name Romboutsia hominis sp. nov. is proposed. The type strain is FRIFI (=DSM 28814=KCTC 15553).

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Keyword(s): Clostridium , human intestine , ileostoma effluent , Peptostreptococcaceae and Romboutsia
© 2018 IUMS
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2018-09-18
2024-04-20
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Romboutsia hominis sp. nov., the first human gut-derived representative of the genus Romboutsia, isolated from ileostoma effluent
Int J Syst Evol Microbiol 68, 3479 (2018); https://doi.org/10.1099/ijsem.0.003012
/content/journal/ijsem/10.1099/ijsem.0.003012
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