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Salinimonas sediminis sp. nov., a piezophilic bacterium isolated from a deep-sea sediment sample from the New Britain Trench, Page 1 of 1
< Previous page | Next page > /docserver/preview/fulltext/ijsem/68/12/3766_ijsem003055-1.gifA cold-adapted, piezophilic, slightly halophilic bacterium, designated as N102T, was isolated from a deep-sea (4700 m) sediment sample collected from the New Britain Trench. Strain N102T was Gram-stain-negative, rod-shaped, oxidase- and catalase-positive, and grew optimally at 28 °C (range, 4–40 °C), pH 7.0–7.5 (range, 6.0–9.0) and 3–4 %(w/v) NaCl (range, 2–15 %). The optimum pressure for growth was 10 MPa with tolerance up to 70 MPa. 16S rRNA gene sequence analysis showed that strain N102T was most closely related to Alteromonas addita R10SW13T (97.2 %), Alteromonas stellipolaris LMG 21861T (97.1 %), Alteromonas gracilis 9a2T (97.1 %), Salinimonas lutimaris DPSR-4T (96.1 %) and Salinimonas chungwhensis BH030046T (95.4 %). Phylogenetic analyses based on 16S rRNA gene, gyrB gene and whole-genome sequences placed strain N102T within the genus Salinimonas . Genomic comparisons based on average nucleotide identity and tetranucleotide signature frequencies corroborated the results of the phylogenetic analyses. The principal fatty acids were summed feature 3 (C16 : 1 ω7c/C16 : 1ω6c), C16 : 0 and summed feature 8 (C18 : 1 ω7c/C18 : 1ω6c). The major respiratory quinone was ubiquinone 8. The predominant polar lipids were phosphatidylethanolamine, phosphatidylglycerol and an unidentified phospholipid. The G+C content of the genomic DNA was 48.2 mol%. On the basis of phenotypic, chemotaxonomic and molecular data, we conclude that strain N102T represents a novel species of the genus Salinimonas , for which the name Salinimonas sediminis sp. nov. is proposed (type strain N102T=MCCC 1K03497T=KCTC 62440T).
The GenBank [/EMBL/DDBJ] accession numbers for the nucleotide sequence reported in this study MH816968 (Salinimonas lutimaris KCTC 23464T, gyrB gene), MG745360 (Salinimonas sediminis N102T, 16SrRNA gene) and CP031769 (Salinimonas sediminis N102T, complete genome).
One supplementary table and six supplementary figures are available with the online version of this article.
© 2018 IUMS | Published by the Microbiology Society
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