%0 Journal Article %A Sun, Cong %A Xu, Lin %A Yu, Xiao-Yun %A Zhao, Zhe %A Wu, Yue-Hong %A Oren, Aharon %A Wang, Chun-Sheng %A Xu, Xue-Wei %T Minwuia thermotolerans gen. nov., sp. nov., a marine bacterium forming a deep branch in the Alphaproteobacteria, and proposal of Minwuiaceae fam. nov. and Minwuiales ord. nov. %D 2018 %J International Journal of Systematic and Evolutionary Microbiology, %V 68 %N 12 %P 3856-3862 %@ 1466-5034 %R https://doi.org/10.1099/ijsem.0.003073 %K Alphaproteobacteria %K Minwuia thermotolerans %K marine %K Minwuiales ord. nov. %K aerobic %K Minwuiaceae fam. nov. %I Microbiology Society, %X Two Gram-stain-negative, strictly aerobic, non-motile, non-spore-forming, rod-shaped bacteria, designated as SY3-15Tand SY3-13, were isolated from a seawater sample of the South China Sea. Colonies were 0.5–1.0 mm in diameter, smooth, circular, convex and translucent after growth on marine agar at 37 °C for 3 days. The strains were found to grow at 20–50 °C (optimum, 42 °C), pH 6.0–8.5 (optimum, pH 6.5–7.5) and with 0.5–6.0 % (w/v) NaCl (optimum, 1.5–2.0 %). Chemotaxonomic analysis showed the sole respiratory quinone to be ubiquinone-10, the major fatty acids (>10 %) were C16 : 0 3-OH, C19 : 0cyclo ω9c, C18 : 1 3-OH and summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c), and the polar lipids were phosphatidylglycerol, two unidentified aminolipids and three unidentified lipids. The DNA G+C content was 67.2–67.4 mol% calculated by genome. The 16S rRNA gene sequences of strains SY3-15T and SY3-13 were identical and related to the genus Lutibaculum with a similarity of 92.1 %. The 16S rRNA gene phylogenetic trees reconstructed with neighbour-joining, maximum-parsimony and minimum-evolution methods showed that the strains constituted a deep and separated branch from other families of Alphaproteobacteria , and the phylogenetic trees based on concatenated 163 protein sequences from genome sequences showed that the clade in which strains SY3-15T and SY3-13 located was separated from the clade of the other orders of Alphaproteobacteria , indicating it may represent a novel family of a novel order. Based on their phenotypic properties and their phylogenetic distinctiveness, we propose strains SY3-15T (=MCCC 1K03467T=KCTC 62335T) and SY3-13 (=MCCC 1K03466=KCTC 62329) to represent a novel species of a novel genus with the name Minwuia thermotolerans gen. nov., sp. nov., and we propose Minwuiaceae fam. nov. and Minwuiales ord. nov. with Minwuia as the type genus. %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003073