@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.003076, author = "Wang, Xiaoxia and Yang, Jing and Lu, Shan and Lai, Xin-He and Jin, Dong and Pu, Ji and Zhang, Gui and Huang, Ying and Zhu, Wentao and Wu, Xiaomin and Liang, Hao and Xu, Jianguo", title = "Nocardioides houyundeii sp. nov., isolated from Tibetan antelope faeces", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2018", volume = "68", number = "12", pages = "3874-3880", doi = "https://doi.org/10.1099/ijsem.0.003076", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003076", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Tibetan antelope", keywords = "wild life", keywords = "Qinghai-Tibet Plateau", keywords = "Nocardioides houyundeii", abstract = "In the present study, we describe two novel Gram-stain-positive, irregular rod-shaped bacterial strains, 78T and 601, that had been isolated from the faeces of Tibetan antelopes at the Hoh Xil Nature Reserve, Qinghai–Tibet Plateau, China. The cells were aerobic, oxidase-negative and catalase-positive. When cultured on brain–heart infusion agar supplemented with 5 % sheep blood, colonies were cream in colour, circular, smooth and convex. Phylogenetic analysis based on the full-length 16S rRNA sequences revealed that type strain 78T and strain 601 belong to the genus Nocardioides, sharing the highest similarity to Nocardioides solisilvae JCM 31492T (98.3 %), Nocardioides gilvus XZ17T (97.4 %) and Nocardioides daejeonensis JCM 16922T (97.4 %). The average nucleotide identity values between the two novel strains and the three closely related type strains of the genus Nocardioides were lower than the 95–96 % threshold. The DNA G+C content of strains 78T and 601 were 71.2 and 71.3 mol% respectively. MK-8 (H4) was the predominant respiratory quinone and ll-2,6-diaminopimelic acid was the diagnostic diamino acid in its cell-wall peptidoglycan. Its polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, an unidentified phospholipid and an unidentified lipid. The main whole-cell sugars were rhamnose, xylose and galactose and the major fatty acids (>10 %) were C17 : 1ω8c, iso-C16 : 0 and C18 : 1ω9c. These data supported the affiliation of strains 78T and 601 to genus Nocardioides. Based on evidence collected from the phenotypic, genotypic and phylogenetic analyses, we propose a novel species named Nocardioides houyundeii sp. nov. The type strain is 78T (=CGMCC 4.7461T=DSM 106424T).", }