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Volume 69, Issue 2

Pseudomonas mangrovi sp. nov., isolated from mangrove soil Free

Abstract

A Gram-stain-negative, aerobic, non-motile, short-rod-shaped bacterium, designated as strain TC11, was isolated from rhizosphere soil of mangrove forest (Kandeliaobovata) in Fugong village, Zhangzhou, Fujian, China. Strain TC11 grew at 15–45 °C (optimum, 35 °C), 0–8 % (w/v) NaCl (optimum, 1 %, w/v) and pH 5.5–9.5 (optimum, pH 7.5). Phylogenetic analyses revealed that strain TC11 belonged to a clade of the genus Pseudomonas and showed the highest sequence similarity of 98.4 % to Pseudomonas fluvialis ASS-1, followed by Pseudomonas oleovorans subsp. oleovorans DSM 1045 (97.9 %), Pseudomonas indoloxydans JCM 14246 (97.7 %), Pseudomonas guguanensis JCM 18416(97.6 %) and Pseudomonas alcaliphila JCM 10630 (97.5 %) on the basis of their 16S rRNA gene sequences. The DNA G+C content was 64.3 mol%. In silico DNA–DNA hybridization and average nucleotide identity values between strain TC11 and the reference strains were 19–22 % and 72–78 %, respectively. Studies based on the three housekeeping genes, rpoB, gyrB and rpoD, further confirmed that strain TC11 is a novel member of the genus Pseudomonas . The major fatty acids of strain TC11were C16 : 0, summed feature 8 (C18 : 1ω6c/C18 : 1ω7c) and summed feature 3 (C16 : 1ω7c/C16 : 1ω6c). The sole isoprenoid quinone was Q-9. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Based on the phenotypic, chemotaxonomic and phylogenetic properties, strain TC11 represents a novel species of the genus Pseudomonas , for which the name Pseudomonas mangrovi sp. nov., is proposed. The type strain is TC11 (=KCTC 62159=MCCC 1K03499).

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Keyword(s): mangrove soil , polyphasic taxonomy and Pseudomonas mangrovi sp. nov.
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2019-01-02
2024-04-16
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Pseudomonas mangrovi sp. nov., isolated from mangrove soil
Int J Syst Evol Microbiol 69, 377 (2019); https://doi.org/10.1099/ijsem.0.003141
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