%0 Journal Article %A Ye, Yanghui %A Chen, Can %A Ren, Yanhu %A Wang, Ruijun %A Zhang, Chongya %A Han, Shuaibo %A Ju, Zhao %A Zhao, Zhu %A Sun, Cong %A Wu, Min %T Pseudomonas mangrovi sp. nov., isolated from mangrove soil %D 2019 %J International Journal of Systematic and Evolutionary Microbiology, %V 69 %N 2 %P 377-383 %@ 1466-5034 %R https://doi.org/10.1099/ijsem.0.003141 %K mangrove soil %K Pseudomonas mangrovi sp. nov. %K polyphasic taxonomy %I Microbiology Society, %X A Gram-stain-negative, aerobic, non-motile, short-rod-shaped bacterium, designated as strain TC11T, was isolated from rhizosphere soil of mangrove forest (Kandeliaobovata) in Fugong village, Zhangzhou, Fujian, China. Strain TC11T grew at 15–45 °C (optimum, 35 °C), 0–8 % (w/v) NaCl (optimum, 1 %, w/v) and pH 5.5–9.5 (optimum, pH 7.5). Phylogenetic analyses revealed that strain TC11T belonged to a clade of the genus Pseudomonas and showed the highest sequence similarity of 98.4 % to Pseudomonas fluvialis ASS-1T, followed by Pseudomonas oleovorans subsp. oleovorans DSM 1045T (97.9 %), Pseudomonas indoloxydans JCM 14246T (97.7 %), Pseudomonas guguanensis JCM 18416T(97.6 %) and Pseudomonas alcaliphila JCM 10630T (97.5 %) on the basis of their 16S rRNA gene sequences. The DNA G+C content was 64.3 mol%. In silico DNA–DNA hybridization and average nucleotide identity values between strain TC11T and the reference strains were 19–22 % and 72–78 %, respectively. Studies based on the three housekeeping genes, rpoB, gyrB and rpoD, further confirmed that strain TC11T is a novel member of the genus Pseudomonas . The major fatty acids of strain TC11Twere C16 : 0, summed feature 8 (C18 : 1ω6c/C18 : 1ω7c) and summed feature 3 (C16 : 1ω7c/C16 : 1ω6c). The sole isoprenoid quinone was Q-9. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Based on the phenotypic, chemotaxonomic and phylogenetic properties, strain TC11T represents a novel species of the genus Pseudomonas , for which the name Pseudomonas mangrovi sp. nov., is proposed. The type strain is TC11T (=KCTC 62159=MCCC 1K03499). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003141