Pseudomonas mangrovi sp. nov., isolated from mangrove soil
Ye, Yanghui and Chen, Can and Ren, Yanhu and Wang, Ruijun and Zhang, Chongya and Han, Shuaibo and Ju, Zhao and Zhao, Zhu and Sun, Cong and Wu, Min,, 69, 377-383 (2019),
doi = https://doi.org/10.1099/ijsem.0.003141,
publicationName = Microbiology Society,
issn = 1466-5026,
abstract= A Gram-stain-negative, aerobic, non-motile, short-rod-shaped bacterium, designated as strain TC11T, was isolated from rhizosphere soil of mangrove forest (Kandeliaobovata) in Fugong village, Zhangzhou, Fujian, China. Strain TC11T grew at 15–45 °C (optimum, 35 °C), 0–8 % (w/v) NaCl (optimum, 1 %, w/v) and pH 5.5–9.5 (optimum, pH 7.5). Phylogenetic analyses revealed that strain TC11T belonged to a clade of the genus Pseudomonas and showed the highest sequence similarity of 98.4 % to Pseudomonas fluvialis ASS-1T, followed by Pseudomonas oleovorans subsp. oleovorans DSM 1045T (97.9 %), Pseudomonas indoloxydans JCM 14246T (97.7 %), Pseudomonas guguanensis JCM 18416T(97.6 %) and Pseudomonas alcaliphila JCM 10630T (97.5 %) on the basis of their 16S rRNA gene sequences. The DNA G+C content was 64.3 mol%. In silico DNA–DNA hybridization and average nucleotide identity values between strain TC11T and the reference strains were 19–22 % and 72–78 %, respectively. Studies based on the three housekeeping genes, rpoB, gyrB and rpoD, further confirmed that strain TC11T is a novel member of the genus Pseudomonas . The major fatty acids of strain TC11Twere C16 : 0, summed feature 8 (C18 : 1ω6c/C18 : 1ω7c) and summed feature 3 (C16 : 1ω7c/C16 : 1ω6c). The sole isoprenoid quinone was Q-9. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Based on the phenotypic, chemotaxonomic and phylogenetic properties, strain TC11T represents a novel species of the genus Pseudomonas , for which the name Pseudomonas mangrovi sp. nov., is proposed. The type strain is TC11T (=KCTC 62159=MCCC 1K03499).,
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