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Volume 69, Issue 3

Rufibacter sediminis sp. nov., isolated from freshwater lake sediment Free

Abstract

A novel Gram-stain-negative, aerobic and rod-shaped bacterial strain, designated H-1, was isolated from the interfacial sediment of Taihu Lake in China and characterized by using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the organism was most closely related to Rufibacter immobilis MCC P1 and Rufibacter tibetensis 1351, with sequence similarities of 98.0 and 97.6 %, respectively. DNA–DNA relatedness between strain H-1 and R. immobilis MCC P1 and R. tibetensis 1351 was 48.8 and 36.6 %, respectively. The major (>5 %) cellular fatty acids were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), C16 : 1ω5c, iso-C15 : 0, summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B) and anteiso-C15 : 0. The polar lipids comprised phosphatidylethanolamine, three unidentified aminophospholipids, an unidentified phospholipid and three unidentified lipids. The major respiratory quinone was menaquinone 7 (MK-7). Genomic DNA G+C content was 49.0 mol%. Based on its physiological, biochemical and chemotaxonomic characteristics, the strain represents a novel species of the genus Rufibacter , for which the name Rufibacter sediminis sp. nov. (type strain H-1=CGMCC 1.16289=NBRC 113030) is proposed.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): polyphasic identification , Rufibacter sediminis , sediment and Taihu Lake
© 2019 IUMS
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2019-01-23
2024-04-19
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Rufibacter sediminis sp. nov., isolated from freshwater lake sediment
Int J Syst Evol Microbiol 69, 662 (2019); https://doi.org/10.1099/ijsem.0.003177
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