1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 69, Issue 2

Meridianimarinicoccus roseus gen. nov., sp. nov., a novel genus of the family Rhodobacteraceae isolated from seawater Free

Abstract

A Gram-stain negative, pink-pigmented, strictly aerobic, non-motile and coccoid-shaped bacterial strain, designated TG-679, was isolated from a deep-sea water sample collected from the South China Sea. Cells of strain TG-679 were catalase- and oxidase-positive, lacked bacteriochlorophyll a and carotenoid. Strain TG-679 was found to grow at 10–40 °C (optimum, 28 °C), pH 5.5–10.0 (optimum, pH 7.5) and with 0.5–2.0 % (w/v) NaCl (optimum, 1.5 %). Chemotaxonomic analysis of strain TG-679 indicated that the sole respiratory quinone was Q-10, the predominant cellular fatty acid was C18 : 1ω7c, and the major polar lipids consisted of phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol, phosphatidylcholine, an unidentified phospholipid and two unidentified glycolipids. The genomic DNA G+C content of strain TG-679 was 65.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain TG-679 constituted a separated branch in the family Rhodobacteraceae . Differential phenotypic properties, together with phylogenetic distinctiveness, demonstrated that strain TG-679 is clearly distinct from any validly published genus. Based on polyphasic taxonomic characterization, strain TG-679 is considered to represent a novel species of a novel genus, for which the name Meridianimarinicoccus roseus gen. nov., sp. nov. is proposed. The type strain of the species is TG-679 (=KCTC 62454=MCCC 1K03496).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Meridianimarinicoccus roseus , Rhodobacteraceae and South China Sea water
© 2019 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.003185
2019-01-03
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/69/2/504.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.003185&mimeType=html&fmt=ahah

References

  1. Garrity G, Brenner DJ, Kreig N, Staley JT. Bergey’s Manual of Systematic Bacteriology, Vol. 2, Part C (The Alpha-, Beta-, Delta-, and Epsilonproteobacteria) New York: Springer; 2005pp. 161–.229
     [Google Scholar]
  2. Parte AC. LPSN-list of prokaryotic names with standing in nomenclature. Nucleic Acids Res 2014; 42:D613–D616 [View Article][PubMed]
     [Google Scholar]
  3. Choi DH, Cho BC. Citreimonas salinaria gen. nov., sp. nov., a member of the Roseobacter clade isolated from a solar saltern. Int J Syst Evol Microbiol 2006; 56:2799–2803 [View Article][PubMed]
     [Google Scholar]
  4. Li S, Tang K, Liu K, Jiao N. Thiobacimonas profunda gen. nov., sp. nov., a member of the family Rhodobacteraceae isolated from deep-sea water. Int J Syst Evol Microbiol 2015; 65:359–364 [View Article][PubMed]
     [Google Scholar]
  5. Yang Y, Sun J, Tang K, Lin D, Li C et al. Ponticoccus lacteus sp. nov. of the family Rhodobacteraceae, isolated from surface seawater. Int J Syst Evol Microbiol 2015; 65:1247–1250 [View Article][PubMed]
     [Google Scholar]
  6. Dong XZ, Cai MY. Common Manual of Systematic Bacteriology Beijing: Science Press; 2001
     [Google Scholar]
  7. Lee MJ, Lee SS. Paracoccus limosus sp. nov., isolated from activated sludge in a sewage treatment plant. Int J Syst Evol Microbiol 2013; 63:1311–1316 [View Article][PubMed]
     [Google Scholar]
  8. Pujalte MJ, Lucena T, Ruvira MA, Arahal DR, Macián MC et al. The family Rhodobacteraceae. The Prokaryotes – Alphaproteobacteria and Betaproteobacteria Berlin: Springer Verlag; 2014 pp. pp.439–.512
     [Google Scholar]
  9. Sun C, Wu C, Su Y, Wang RJ, Fu GY et al. Hyphococcus flavus gen. nov., sp. nov., a novel alphaproteobacterium isolated from deep seawater. Int J Syst Evol Microbiol 2017; 67:4024–4031 [View Article][PubMed]
     [Google Scholar]
  10. Weisburg WG, Barns SM, Pelletier DA, Lane DJ. 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 1991; 173:697–703 [View Article][PubMed]
     [Google Scholar]
  11. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012; 62:716–721 [View Article][PubMed]
     [Google Scholar]
  12. Zhang XQ, Sun C, Wang CS, Zhang X, Zhou X et al. Sinimarinibacterium flocculans gen. nov., sp. nov., a gammaproteobacterium from offshore surface seawater. Int J Syst Evol Microbiol 2015; 65:3541–3546 [View Article][PubMed]
     [Google Scholar]
  13. Tamura K, Peterson D, Peterson N, Stecher G, Nei M et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011; 28:2731–2739 [View Article][PubMed]
     [Google Scholar]
  14. Simpson JT, Wong K, Jackman SD, Schein JE, Jones SJ et al. ABySS: a parallel assembler for short read sequence data. Genome Res 2009; 19:1117–1123 [View Article][PubMed]
     [Google Scholar]
  15. Parks DH, Imelfort M, Skennerton CT, Hugenholtz P, Tyson GW. CheckM: assessing the quality of microbial genomes recovered from isolates, single cells, and metagenomes. Genome Res 2015; 25:1043–1055 [View Article][PubMed]
     [Google Scholar]
  16. Lee I, Ouk Kim Y, Park SC, Chun J. OrthoANI: an improved algorithm and software for calculating average nucleotide identity. Int J Syst Evol Microbiol 2016; 66:1100–1103 [View Article][PubMed]
     [Google Scholar]
  17. Meier-Kolthoff JP, Auch AF, Klenk HP, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 2013; 14:60 [View Article][PubMed]
     [Google Scholar]
  18. Zhong ZP, Liu Y, Liu HC, Wang F, Zhou YG et al. Roseibium aquae sp. nov., isolated from a saline lake. Int J Syst Evol Microbiol 2014; 64:2812–2818 [View Article][PubMed]
     [Google Scholar]
  19. Trüper HG, Pfennig N. Isolation of members of the families Chromatiaceae and Chlorobiaceae. In Starr MP, Stolp H, Trüper HG, Balows A, Schlegel HG et al. (editors) The Prokaryotes:a Handbook on Habitats, Isolation, and Identification of Bacteria Berlin: Springer; 1981 pp. 279–289
     [Google Scholar]
  20. Smibert RM, Krieg NR. Phenotypic characterization. Methods for General and Molecular Bacteriology Washington, DC: American Society of Microbiology; 1994 pp. 607–654
     [Google Scholar]
  21. Sun C, Huo YY, Liu JJ, Pan J, Qi YZ et al. Thalassomonas eurytherma sp. nov., a marine proteobacterium. Int J Syst Evol Microbiol 2014; 64:2079–2083 [View Article][PubMed]
     [Google Scholar]
  22. Zhang XQ, Wu YH, Zhou X, Zhang X, Xu XW et al. Parvularcula flava sp. nov., an alphaproteobacterium isolated from surface seawater of the South China Sea. Int J Syst Evol Microbiol 2016; 66:3498–3502 [View Article][PubMed]
     [Google Scholar]
  23. Wu XY, Zheng G, Zhang WW, Xu XW, Wu M, Xy W, Xw X et al. Amphibacillus jilinensis sp. nov., a facultatively anaerobic, alkaliphilic bacillus from a soda lake. Int J Syst Evol Microbiol 2010; 60:2540–2543 [View Article][PubMed]
     [Google Scholar]
  24. Zhong ZP, Liu Y, Wang F, Zhou YG, Liu HC et al. Planktosalinus lacus gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from a salt lake. Int J Syst Evol Microbiol 2016; 66:2084–2089 [View Article][PubMed]
     [Google Scholar]
  25. Collins MD, Pirouz T, Goodfellow M, Minnikin DE. Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 1977; 100:221–230 [View Article][PubMed]
     [Google Scholar]
  26. Komagata K, Suzuki K. Lipids and cell-wall analysis in bacterial systematics. Methods Microbiol 1988; 19:161–207
     [Google Scholar]
  27. Kates M, Lipidology T. Isolation, Analysis and Identification of Lipids, 2nd ed. Amsterdam: Elsevier; 1986
     [Google Scholar]
  28. Tindall BJ. Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Lett 1990; 66:199–202 [View Article]
     [Google Scholar]
  29. Chun J, Oren A, Ventosa A, Christensen H, Arahal DR et al. Proposed minimal standards for the use of genome data for the taxonomy of prokaryotes. Int J Syst Evol Microbiol 2018; 68:461–466 [View Article][PubMed]
     [Google Scholar]
  30. Richter M, Rosselló-Móra R. Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci USA 2009; 106:19126–19131 [View Article][PubMed]
     [Google Scholar]
  31. Wayne LG, Moore WEC, Stackebrandt E, Kandler O, Colwell RR et al. Report of the Ad Hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Evol Microbiol 1987; 37:463–464 [View Article]
     [Google Scholar]
  32. Konstantinidis KT, Tiedje JM. Towards a genome-based taxonomy for prokaryotes. J Bacteriol 2005; 187:6258–6264 [View Article][PubMed]
     [Google Scholar]
  33. Srinivas A, Vinay Kumar B, Divya Sree B, Tushar L, Sasikala C et al. Rhodovulum salis sp. nov. and Rhodovulum viride sp. nov., phototrophic Alphaproteobacteria isolated from marine habitats. Int J Syst Evol Microbiol 2014; 64:957–962 [View Article][PubMed]
     [Google Scholar]
  34. Divyasree B, Lakshmi KV, Bharti D, Sasikala C, Ramana C. Rhodovulum aestuarii sp. nov., isolated from a brackish water body. Int J Syst Evol Microbiol 2016; 66:165–171 [View Article][PubMed]
     [Google Scholar]
  35. Ramaprasad EV, Tushar L, Dave B, Sasikala C, Ramana CV et al. Rhodovulum algae sp. nov., isolated from an algal mat. Int J Syst Evol Microbiol 2016; 66:3367–3371 [View Article][PubMed]
     [Google Scholar]
  36. Nupur P, Srinivas TN, Takaichi S, Anil Kumar P. Rhodovulum mangrovi sp. nov., a phototrophic alphaproteobacterium isolated from a mangrove forest sediment sample. Int J Syst Evol Microbiol 2014; 64:3168–3173 [View Article][PubMed]
     [Google Scholar]
  37. Romanenko LA, Tanaka N, Svetashev VI, Kalinovskaya NI. Poseidonocella pacifica gen. nov., sp. nov. and Poseidonocella sedimentorum sp. nov., novel alphaproteobacteria from the shallow sandy sediments of the Sea of Japan. Arch Microbiol 2012; 194:113–121 [View Article][PubMed]
     [Google Scholar]
  38. Tomonori S, Yasutaka M, Manabu T, Yukimasa N. Roseivivax halodurans gen nov., sp. now and Roseivivax halotolerans sp. nov., aerobic bacteriochlorophyll-containing bacteria isolated from a saline lake. Int J Syst Bacteriology 1999; 49:629–634
     [Google Scholar]
  39. Park S, Kang SJ, Oh TK, Yoon JH. Roseivivax lentus sp. nov., isolated from a tidal flat sediment, and emended description of the genus Roseivivax Suzuki et al. 1999. Int J Syst Evol Microbiol 2010; 60:1113–1117 [View Article][PubMed]
     [Google Scholar]
  40. Dai XF, Shi XC, Gao X, Liu JL, Zhang XH et al. Roseivivax marinus sp. nov., isolated from deep water. Int J Syst Evol Microbiol 2014; 64:2540–2544
     [Google Scholar]
  41. Wu YH, Meng FX, Xu L, Zhang XQ, Wang CS et al. Roseivivax pacificus sp. nov., isolated from deep-sea sediment. Int J Syst Evol Microbiol 2013; 63:4574–4579 [View Article][PubMed]
     [Google Scholar]
  42. Zhang YQ, Lee JC, Park DJ, Lu XX, Mou XZ et al. Roseivivax roseus sp. nov., an alphaproteobacterium isolated from a solar saltern soil sample. Int J Syst Evol Microbiol 2014; 64:1743–1746 [View Article][PubMed]
     [Google Scholar]
  43. Wang B, Tan T, Shao Z. Roseovarius pacificus sp. nov., isolated from deep-sea sediment. Int J Syst Evol Microbiol 2009; 59:1116–1121 [View Article][PubMed]
     [Google Scholar]
  44. Jia X, Kim HR, Jia B, Jeon HH, Baek K et al. Roseovarius confluentis sp. nov., isolated from estuary sediment. Int J Syst Evol Microbiol 2017; 67:346–351 [View Article][PubMed]
     [Google Scholar]
  45. Castro DJ, Cerezo I, Sampedro I, Martínez-Checa F. Roseovarius ramblicola sp. nov., a moderately halophilic bacterium isolated from saline soil in Spain. Int J Syst Evol Microbiol 2018; 68:1851–1856 [View Article][PubMed]
     [Google Scholar]
  46. Wang NN, Liu ZY, Jiang LX, Li YX, Du ZJ et al. Roseovarius salinarum sp. nov., isolated from a marine solar saltern. Int J Syst Evol Microbiol 2018; 68:1986–1991 [View Article][PubMed]
     [Google Scholar]
  47. Oh KH, Choi WC, Jung YT, Kang SJ, Oh TK et al. Tropicimonas aquimaris sp. nov., isolated from seawater, and emended description of the genus Tropicimonas Harwati et al. 2009. Int J Syst Evol Microbiol 2012; 62:688–692 [View Article][PubMed]
     [Google Scholar]
  48. Oh M, Kim JH, Kim W. Tropicimonasarenosa sp. nov., isolated from marine sand. Int J Syst Evol Microbiol 2016; 66:5514–5518 [View Article][PubMed]
     [Google Scholar]
  49. Shin NR, Roh SW, Kim MS, Yun B, Whon TW et al. Tropicimonas sediminicola sp. nov., isolated from marine sediment. Int J Syst Evol Microbiol 2012; 62:2424–2429 [View Article][PubMed]
     [Google Scholar]
  50. Lee K, Choo YJ, Giovannoni SJ, Cho JC. Maritimibacter alkaliphilus gen. nov., sp. nov., a genome-sequenced marine bacterium of the Roseobacter clade in the order Rhodobacterales. Int J Syst Evol Microbiol 2007; 57:1653–1658 [View Article][PubMed]
     [Google Scholar]
  51. Zhong ZP, Liu Y, Zhou YG, Liu HC, Wang F et al. Maritimibacter lacisalsi sp. nov., isolated from a salt lake, and emended description of the genus Maritimibacter Lee,. Int J Syst Evol Microbiol 2007; 2015:3462–3468
     [Google Scholar]
  52. Hansen TA, Veldkamp H. Rhodopseudomonas sulfidophila, nov. spec., a new species of the purple nonsulfur bacteria. Arch Mikrobiol 1973; 92:45–58 [View Article][PubMed]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.003185
Loading
Meridianimarinicoccus roseus gen. nov., sp. nov., a novel genus of the family Rhodobacteraceae isolated from seawater
Int J Syst Evol Microbiol 69, 504 (2019); https://doi.org/10.1099/ijsem.0.003185
/content/journal/ijsem/10.1099/ijsem.0.003185
/content/journal/ijsem/10.1099/ijsem.0.003185
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error