RT Journal Article SR Electronic(1) A1 Parshina, Sofiya Nikolaevna A1 Strepis, Nikolaos A1 Aalvink, Steven A1 Nozhevnikova, Alla N. A1 Stams, Alfons J. M. A1 Sousa, Diana Z.YR 2019 T1 Trichococcus shcherbakoviae sp. nov., isolated from a laboratory-scale anaerobic EGSB bioreactor operated at low temperature JF International Journal of Systematic and Evolutionary Microbiology, VO 69 IS 2 SP 529 OP 534 DO https://doi.org/10.1099/ijsem.0.003193 PB Microbiology Society, SN 1466-5034, AB A new species of the genus Trichococcus , strain Art1T, was isolated from a psychrotolerant syntrophic propionate-oxidizing consortium, obtained before from a low-temperature EGSB reactor fed with a mixture of VFAs (acetate, propionate and butyrate). The 16S rRNA gene sequence of strain Art1T was highly similar to those of other Trichococcus species (99.7–99.9 %) but digital DNA–DNA hybridization values were lower than those recommended for the delineation of a novel species, indicating that strain Art1T is a novel species of the genus Trichococcus . Cells of strain Art1T are non-motile cocci with a diameter of 0.5–2.0 µm and were observed singularly, in pairs, short chains and irregular conglomerates. Cells of Art1T stained Gram-positive and produced extracellular polymeric substances . Growth was optimal at pH 6–7.5 and cells could grow in a temperature range of from −2 to 30 °C (optimum 25–30 °C). Strain Art1T can degrade several carbohydrates, and the main products from glucose fermentation are lactate, acetate, formate and ethanol. The genomic DNA G+C content of strain Art1T is 46.7 %. The major components of the cellular fatty acids are C16 : 1 ω9c, C16 : 0 and C18 : 1 ω9c. Based on genomic and physiological characteristics of strain Art1T, a new species of the genus Trichococcus, Trichococcus shcherbakoviae, is proposed. The type strain of Trichococcus shcherbakoviae is Art1T (=DSM 107162T = VKM B–3260T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003193