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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 69, Issue 2

Nibricoccus aquaticus gen. nov., sp. nov., a new genus of the family Opitutaceae isolated from hyporheic freshwater Free

Abstract

A yellow-coloured, Gram-strain-negative, non-motile, cocci-shaped, strictly aerobic bacterium, designated HZ-65, was isolated from hyporheic freshwater in the Republic of Korea. Strain HZ-65 grew at 15–37 °C (optimum, 25–30 °C), pH 5.5–9.0 (optimum, pH 7.0) and 0–0.5 % NaCl (w/v; optimum at 0 % NaCl). Phylogenetic analysis based on the 16S rRNA gene showed that strain HZ-65 is a member of family Opitutaceae and is closely related to Opitutus terrae PB90-1 (94.0 % similarity), Cephaloticoccus primus CAG34 (93.0 %), and Cephaloticoccus capnophilus CV41 (92.7 %), while the similarities to other Opitutaceae -type strains were lower than 90.0 %. The DNA G+C content was 62.2 mol% and the quinone present was menaquinone-7. The predominant fatty acids were iso-C14 : 0, anteiso-C15 : 0, C16 : 0, and iso-C16 : 0, representing 70 % of the total fatty acids. The major polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Analysis of the HZ-65 genome revealed the presence of 300 genes that are involved in carbohydrate-active enzymes, which indicates the metabolic potential to degrade polysaccharides. The phenotypic, chemotaxonomic, genetic, and phylogenetic properties suggest that strain HZ-65 represents a novel species in a new genus within the family Opitutaceae, for which the name Nibricoccus aquaticus gen. nov., sp. nov., is proposed. The type strain of Nibricoccus aquaticus is HZ-65 (KACC 19333=NBRC 112907).

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Keyword(s): class Opitutae , hyporheic freshwater , Nibricoccus , Nibricoccus aquaticus , phylum Verrucomicrobia and polyphasic taxonomy
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2018-12-21
2024-04-20
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Nibricoccus aquaticus gen. nov., sp. nov., a new genus of the family Opitutaceae isolated from hyporheic freshwater
Int J Syst Evol Microbiol 69, 552 (2019); https://doi.org/10.1099/ijsem.0.003198
/content/journal/ijsem/10.1099/ijsem.0.003198
/content/journal/ijsem/10.1099/ijsem.0.003198
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