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Zavarzinia aquatilis sp. nov., isolated from a freshwater river
Abstract
A Gram-stain-negative, strictly aerobic, catalase-positive and oxidase-positive bacterium, designated strain HR-AST, was isolated from a water sample of the Han River located in the Republic of Korea. Cells were motile rods with a polar flagellum. Growth was observed at 5–35 °C (optimum of 25 °C) and pH 6–8 (optimum of pH 7) and in the presence of 0–2 % (w/v) NaCl (optimum of 0 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain HR-AST formed a tight phylogenic lineage with Zavarzinia compransoris LMG 5821T. Strain HR-AST was most closely related to Z. compransoris LMG 5821T with a 98.7 % 16S rRNA gene sequence similarity and had very low similarities (below 91.0 %) to other type strains with validly published names. Average nucleotide identity and in silico DNA–DNA hybridization values between strain HR-AST and Z. compransoris DSM 1231T were 80.4 and 23.1 %, respectively. Strain HR-AST contained ubiquinone-10 as the major quinone and homospermidine and putrescine as the major polyamines. The major fatty acids were summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c), C16 : 0 and C18 : 1 2-OH. Strain HR-AST contained diphosphatidylglycerol, an unidentified aminolipid and two unidentified phospholipids as major polar lipids. The DNA G+C content of strain HR-AST was 67.2 mol%. Based on the genotypic, chemotaxonomic and phenotypic analyses, strain HR-AST represents a novel species of the genus Zavarzinia , for which the name Zavarzinia aquatilis sp. nov. is proposed. The type strain is HR-AST (=KACC 19412T=JCM 32263T).
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