RT Journal Article SR Electronic(1) A1 Wei, Yuli A1 Cao, Junwei A1 Mao, Haiyan A1 Pei, Jiahao A1 Liu, Rulong A1 Fang, JiasongYR 2019 T1 Marinomonas shanghaiensis sp. nov., isolated from the junction between an ocean and a freshwater lake JF International Journal of Systematic and Evolutionary Microbiology, VO 69 IS 3 SP 805 OP 810 DO https://doi.org/10.1099/ijsem.0.003241 PB Microbiology Society, SN 1466-5034, AB A Gram-stain-negative, motile with single polar flagellum, rod-shaped bacterium, designated as strain DSL-35T, was isolated from the location where the ocean and Dishui lake meet at Shanghai on the East China Sea and characterized phylogenetically and phenotypically. Optimal growth occurred at 35 °C (range, 4–40 °C), pH 8 pH 5–11) and with 3–4 % (w/v) NaCl (0–12 %). Phylogenetic analysis based on its 16S rRNA gene sequence showed that strain DSL-35T was related to members of the genus Marinomonas and shared the highest sequence identities with Marinomonas arctica 328T (98.0 %), Marinomonas hwangdonensis HDW-15T (97.5 %) and Marinomonas rhizomae IVIA-Po-145T (97.2 %). The 16S rRNA gene sequence identities between strain DSL-35T and other members of the genus Marinomonas were below 96.8 %. The digital DNA–DNA hybridization values between strain DSL-35T and the three type strains, Marinomonas . arctica 328T, M. rhizomae HDW-15T and M. rhizomae IVIA-Po-145T, were 30.9±2.4 %, 21.7±2.2% and 22±2.3 %, respectively. The average nucleotide identity values between strain DSL-35T and the three type strains were 87.6 %, 84.6 and 84.2 %, respectively. The predominant ubiquinone was Q-8. The major polar lipids were phosphatidylethanolamine and phosphatidylglycerol. The predominant cellular fatty acids of strain DSL-35T were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c; 40.0 %), C16 : 0 (22.5 %), summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c; 11.2 %), summed feature 2 (C14 : 0 3-OH and/or iso I C16 : 1; 7.2 %), C14 : 0 (6.8 %) and C12 : 0 (5.2 %). The G+C content of the genomic DNA was 44.5 mol%. The combined genotypic and phenotypic data indicated that strain DSL-35T represents a novel species of the genus Marinomonas , for which the name Marinomonas shanghaiensis sp. nov. is proposed, with the type strain DSL-35T (=KCTC 62646T=MCCC 1K03535T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003241