@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.003263, author = "Li, Yan-Qiong and Narsing Rao, Manik Prabhu and Zhang, Hui and Guo, Yan-Mei and Dong, Zhou-Yan and Alkhalifah, Dalal Hussien M. and Hozzein, Wael N. and Xiao, Min and Li, Wen-Jun", title = "Description of Sphingomonas mesophila sp. nov., isolated from Gastrodia elata Blume", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2019", volume = "69", number = "4", pages = "1030-1034", doi = "https://doi.org/10.1099/ijsem.0.003263", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003263", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "genome analysis", keywords = "Sphingomonas mesophila", keywords = "Gastrodia elataBlume", keywords = "endophyte", abstract = "A Gram-staining-negative, strictly aerobic, non-motile strain, SYSUP0001T, was isolated from tubers of Gastrodia elata Blume. The 16S rRNA gene sequence result indicated that SYSUP0001T represents a member of the genus Sphingomonas , with the highest sequence similarity (97.7 %) to the type strain of Sphingomonas ginsengisoli . SYSUP0001T grew at 14–37 °C and pH 6–8, with optimum growth at 28 °C and pH 7. Tolerance to NaCl was up to 3 % (w/v) with optimum growth in the absence of NaCl. The respiratory quinone was Q-10. The major fatty acids were C18 : 1ω7c, Summed feature 3 (C16 : 1ω7c/C16 : 1ω6c), and C16 : 0. The polar lipids were diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), sphingoglycolipid (SGL), phosphatidylcholine (PC) and four unidentified polar lipids (L). The DNA G+C content was 67.5 %. The average nucleotide identity (ANI) values between SYSUP0001T and closely related members of the genus Sphingomonas were below the cut-off level (95–96 %) for species delineation. On the basis of the phenotypic, phylogenetic and chemotaxonomic characterizations, SYSUP0001T represents a novel species of the genus Sphingomonas , for which the name Sphingomonasmesophila sp. nov. is proposed. The type strain is SYSUP0001T (=KCTC 62179 T=CGMCC 1.16462T).", }