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Volume 69, Issue 4

sp. nov., a thiosulfate oxidizer isolated from activated sludge Free

Abstract

A Gram-stain-negative, motile bacterium, designated strain YE3, was isolated from activated sludge obtained from a municipal wastewater treatment plant in Daejeon Metropolitan City, Republic of Korea. The cells were oxidase- and catalase-positive, and grew under aerobic conditions at 10–40 °C (optimum, 30 °C), with 1.0–8.0 % (w/v) NaCl (1.0 %) and at pH 5.5–9.0 (pH 7.0). Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain YE3 was most closely related to KACC 14927 (98.2 % sequence similarity) and KCTC 22046 (98.0 %). DNA–DNA relatedness values for strain YE3 and KACC 14927, KCTC 22046 and KCTC 22455 were 28.7±2.27 %, 21.3±1.16 %, and 14.0±0.67 %, respectively. The genomic G+C content of the type strain YE3 was 59.3 mol%, as determined by whole-genome sequencing. The dominant fatty acids were C (39.2 %) and Ccyclo (37.5 %). The major polar lipids of strain YE3 were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. Two aminophospholipids and four unidentified lipids were also detected. Furthermore, strain YE3 was able to oxidize thiosulfate under heterotrophic conditions. Based on the phenotypic, genotypic, chemotaxonomic and phylogenetic analyses, strain YE3 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is YE3 (=KCTC 62737=NBRC 113113).

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Keyword(s): novel species , Pusillimonas thiosulfatoxidans , sludge and thiosulfate oxidizer
Funding
This study was supported by the:
  • National Research Foundation of Korea (KR) (Award 2018R1C1B6006762)
© 2019 IUMS
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2019-03-05
2024-04-20
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Pusillimonas thiosulfatoxidans sp. nov., a thiosulfate oxidizer isolated from activated sludge
Int J Syst Evol Microbiol 69, 1041 (2019); https://doi.org/10.1099/ijsem.0.003266
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