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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 69, Issue 4

Comparative genomics of and : Reclassification of as comb. nov. Free

Abstract

Previous analyses based on 16S rRNA and genes indicated that and were closely related to each other and formed a monophyletic cluster independent of the related species. To clarify the relationship of these two species, we determined the genome sequence of JCM 31932 and compared it with that already sequenced for Marseille-P3237. Phylogenetic trees based on the concatenated 37 single-copy ribosomal proteins or RpoB robustly supported the relationship observed in the previous studies. Digital DNA–DNA hybridization and average nucleotide identity (ANI) values between JCM 31932 and Marseille-P3237 were 32.6 and 87.8 %, respectively, indicating that JCM 31932 and Marseille-P3237 are independent species. Alignment fraction and ANI values between the two genomes were 0.75 and 88.84 %, respectively, thus indicating that the two species should be classified into the same genus. The number of putative orthologous genes shared between the two genomes was 1321, which was significantly larger than those (482–928) reported between Marseille-P3237 and other closely related species. In addition, the genome of JCM 31932 had a high degree of synteny conservation with that of Marseille-P3237. On the basis of these findings, we propose that should be reclassified as comb. nov.; the type strain is Marseille-P3237 (=JCM 33000=CCUG 71182).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Atopobiaceae , Comparative genomics , Libanicoccus massiliensis and Parolsenella catena
Funding
This study was supported by the:
  • Japan Agency for Medical Research and Development (Award JP18gm6010007)
  • RIKEN (Award none)
  • Japan Agency for Medical Research and Development (Award JP18gm1010006)
  • Japan Society for the Promotion of Science (Award 17H01447)
© 2019 IUMS
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2019-02-14
2024-04-25
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Comparative genomics of Parolsenella catena and Libanicoccus massiliensis: Reclassification of Libanicoccus massiliensis as Parolsenella massiliensis comb. nov.
Int J Syst Evol Microbiol 69, 1123 (2019); https://doi.org/10.1099/ijsem.0.003283
/content/journal/ijsem/10.1099/ijsem.0.003283
/content/journal/ijsem/10.1099/ijsem.0.003283
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