1887

Abstract

A Gram-stain-negative, rod-shaped, motile bacterial strain, designated 3-5-3, was isolated from maize-cultivated soil artificially contaminated with cadmium, in Nanyang, Henan Province, China. Strain 3-5-3 was oxidase- and catalase-positive. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 3-5-3 was affiliated with the genus and most closely related to MH2 (96.5 % similarity). The average nucleotide identity and digital DNA–DNA hybridization values between 3-5-3 and the closely related species ranged 69.4–84.5 % and 18.1–18.4 %. The genomic G+C content was 53.8 mol%. Anteiso-C was the major fatty acid and MK-7 was the only menaquinone. The diamino acid in the cell-wall peptidoglycan contains -diaminopimelic acid. The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, two unidentified glycolipids, two unidentified aminolipids, one unidentified phospholipid, one unidentified phosphoaminolipid and six unidentified lipids. On the basis of the results obtained in this study, strain 3-5-3 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 3-5-3 (=CGMCC 1.13686=KCTC 33998).

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 41601332)
  • National Natural Science Foundation of China (CN) (Award 41401275)
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2019-02-15
2024-03-28
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