%0 Journal Article %A Intra, Bungonsiri %A Euanorasetr, Jirayut %A Také, Akira %A Inahashi, Yuki %A Mori, Mihoko %A Panbangred, Watanalai %A Matsumoto, Atsuko %T Saccharopolyspora rhizosphaerae sp. nov., an actinomycete isolated from rhizosphere soil in Thailand %D 2019 %J International Journal of Systematic and Evolutionary Microbiology, %V 69 %N 5 %P 1299-1305 %@ 1466-5034 %R https://doi.org/10.1099/ijsem.0.003307 %K Saccharopolyspora rhizosphaerae sp. nov. %K rhizosphere soil %I Microbiology Society, %X A novel actinomycete, designated as strain H219T, was isolated from rhizosphere soil collected under an Elephant ear plant (Colocasiaesculenta) in Bangkok, Thailand. Strain H219T was characterised using a polyphasic approach. Phylogenetic analysis of the 16S rRNA gene sequences revealed that this isolate was most closely related to Saccharopolyspora tripterygii JCM 32123T (97.6 %), Saccharopolyspora dendranthemae NBRC 108675T (97.5 %) and Saccharopolyspora flava NBRC 16345T (97.5 %). However, DNA–DNA hybridization analyses showed a low relatedness in the range of 39–48 % between the novel isolate and the above closely related strains. The cell-wall peptidoglycan of strain H219T contained meso-diaminopimelic acid. The diagnostic whole-cell sugars consisted of arabinose and galactose. The cellular fatty acid profile mainly comprised iso-C16 : 0, anteiso-C17 : 0, iso-C15 : 0, and 10-methyl C17 : 0. The major menaquinone was MK-9(H4). The detected phospholipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylethanolamine-containing hydroxylated fatty acids and an unknown phospholipid. The DNA G+C content was 70.6 mol%. Strain H219T represented chemotaxonomic and morphological characteristics that were consistent with members of the genus Saccharopolyspora . However, strain H219T could be distinguished from closely related strains by several phenotypic properties. Based on the data from the polyphasic studies, we propose that strain H219T is a novel species within the genus Saccharopolyspora , Saccharopolyspora rhizosphaerae sp. nov. The type strain is H219T (=TBRC 8564T=NBRC 113388T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003307