@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.003320, author = "Anandham, Rangasamy and Heo, Jun and Krishnamoorthy, Ramasamy and SenthilKumar, Murugaiyan and Gopal, Nellaiappan Olgaganathan and Kim, Soo-Jin and Kwon, Soon-Wo", title = "Azospirillum ramasamyi sp. nov., a novel diazotrophic bacterium isolated from fermented bovine products", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2019", volume = "69", number = "5", pages = "1369-1375", doi = "https://doi.org/10.1099/ijsem.0.003320", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003320", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Alphaproteobacteria", keywords = "Azospirillum ramasamyi sp. nov", keywords = "fermented bovine products", keywords = "organic fertilizer", keywords = "diazotroph", abstract = "A Gram-stain-negative, rod-shaped, aerobic bacterium, designated M2T2B2T, was isolated from fermented bovine products in Suwon, Republic of Korea. The strain displayed growth at 15–45 °C (optimum, 28–30 °C), pH 6.0–10.0 (pH 7.0) and 0–2 % (w/v) NaCl (0 %). Colonies were light pink-coloured, round and convex. The cells were positive for oxidase and weakly positive for catalase. The major fatty acids in whole cells of strain M2T2B2T were summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c), followed by summed feature 3 (C16 : 1 ω7c/C16 : 1 ω6c), summed feature 2 (C12 : 0 aldehyde/unidentified 10.928/C14 : 0 3-OH/iso-C16 : 1 I), C16 : 0, C18 : 1 2-OH, C16 : 0 3-OH and C17 : 1 ω6c. The polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, and three unidentified aminolipids. Ubiquinone 10 was the predominant ubiquinone. The DNA G+C content was 68.0 mol%. The strain could fix atmospheric nitrogen, which was evaluated by the acetylene reduction assay. Further, whole genome sequence analysis revealed the presence of a nif gene cluster. Strain M2T2B2T showed the highest 16S rRNA, rpoD and nifH gene sequence similarity to members of the genus Azospirillum , and showed 97.6 % 16S rRNA gene sequence similarity to Azospirillum oryzae COC8T. The phenotypic, phylogenetic and genomic analyses support the proposal of strain M2T2B2T as being a novel species of the genus Azospirillum , for which the name Azospirillum ramasamyi sp. nov. is proposed. The type strain is M2T2B2T (=KACC 14063T=NBRC 106460T).", }