@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.003333, author = "Wang, Xiaoxia and Yang, Jing and Lu, Shan and Lai, Xin-He and Jin, Dong and Pu, Ji and Niu, Lina and Zhu, Wentao and Liang, Junrong and Huang, Ying and Wang, Bin and Wu, Xiaomin and Liang, Hao and Xu, Jianguo", title = "Paraliobacillus zengyii sp. nov., a slightly halophilic and extremely halotolerant bacterium isolated from Tibetan antelope faeces", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2019", volume = "69", number = "5", pages = "1426-1432", doi = "https://doi.org/10.1099/ijsem.0.003333", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003333", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Tibetan antelope", keywords = "Qinghai-Tibet Plateau", keywords = "Paraliobacillus zengyii sp. nov.", abstract = "Two rod-shaped, slightly halophilic and extremely halotolerant bacterial strains (X-1125T and X-1174), which were Gram-stain-positive, facultatively anaerobic and motile with peritrichous flagella, were isolated from the faeces of Tibetan antelopes. Their optimal temperature, NaCl concentration and pH for growth were 28 °C, 3 % (w/v) NaCl and pH 7.5, respectively. Based on the results of 16S rRNA gene sequences, and phylogenetic and phylogenomic analyses, their nearest phylogenetic neighbours were Paraliobacillussediminis KCTC 33762T (98.4 % similarity), Paraliobacillusquinghaiensis CGMCC 1.6333T (96.9 %) and Paraliobacillusryukyuensis NBRC 100001T (95.9 %) while the 16S rRNA genes of strains X-1125T and X-1174 were highly similar (99.7 %) to each other. The polar lipids comprised diphosphatidylglycerol, two unidentified phospholipids and four unidentified lipids. MK-7 was the sole menaquinone (100 %). The cell wall contained alanine, glycine, glutamic acid and meso-diaminopimelic acid. The major fatty acids (>9 %) were anteiso-C15 : 0, anteiso-C17 : 0 and C16 : 1ω11c. The in silico DNA–DNA hybridization value between strains X-1125T and X-1174 was 97.8 % (well above the species threshold), but their values were lower than the 70 % threshold with the three closely related type strains. Strains X-1125T and X-1174 had DNA G+C contents (mol%) of 35.2 and 35.1 %, respectively. Based on the presented data, strains X-1125T and X-1174 hereby represent a novel species of the genus Paraliobacillus , for which the name Paraliobacillus zengyii sp. nov. is proposed. The type strain is X-1125T (=DSM 107811T=CGMCC 1.16464T).", }