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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 69, Issue 5

Staphylospora marina gen. nov., sp. nov., a novel member of the family Thermoactinomycetaceae, isolated from a deep-sea hydrothermal vent in the Pacific Ocean Free

Abstract

A novel bacterium, designated SCSIO 07575, was isolated from a deep-sea hydrothermal sediment sample collected from the western Pacific Ocean. Growth at 65 °C was observed, but not at 70°C or below 37 °C. The optimum conditions for growth were at 55–65 °C, pH 7.0 and in the presence of 2 % (w/v) NaCl. Strain SCSIO 07575 showed filamentous growth. Unstable formation of white aerial mycelia was observed, which disappeared after several times’ subculture. Abundant substrate mycelia were observed with grape-like spores. No soluble pigment was observed. Phylogenetic analysis of 16S rRNA gene sequences showed that SCSIO 07575 belonged to the family Thermoactinomycetaceae and formed a distinct clade in the phylogenetic tree. The cell-wall peptidoglycan contained meso-diaminopimelic acid. Whole-cell hydrolysates contained ribose, xylose, glucose and galactose. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unidentified aminophospholipid and two unidentified phospholipids. The predominant menaquinone was MK-7. Major fatty acids were iso-C15 : 0, iso-C17 : 0 and iso-C16 : 0. Based on the whole genome sequence analysis, the genome size was 2 751 094 bp with a DNA G+C value of 57.2 mol%, including one circular chromosome and one plasmid. On the basis of polyphasic data, strain SCSIO 07575 represented a novel species of a new genus within the family Thermoactinomycetaceae , for which the name Staphylospora gen. nov. is proposed with the type species Staphylospora marina sp. nov. and the type strain SCSIO 07575 (=DSM 106793=CGMCC 1.15879).

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Keyword(s): deep-sea sediment , hydrothermal vent , Staphylospora marina gen. nov., sp. nov. , the western Pacific Ocean and thermophilic
© 2019 The Authors
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2019-03-18
2024-04-20
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Staphylospora marina gen. nov., sp. nov., a novel member of the family Thermoactinomycetaceae, isolated from a deep-sea hydrothermal vent in the Pacific Ocean
Int J Syst Evol Microbiol 69, 1452 (2019); https://doi.org/10.1099/ijsem.0.003339
/content/journal/ijsem/10.1099/ijsem.0.003339
/content/journal/ijsem/10.1099/ijsem.0.003339
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