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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 69, Issue 5

Dyella dinghuensis sp. nov. and Dyella choica sp. nov., isolated from forest soil Free

Abstract

Two aerobic, Gram-stain-negative, non-motile, rod-shaped bacterial strains, designated as DHOA06 and 4 M-K27, were isolated from soil samples collected from the forest of Dinghushan Biosphere Reserve, Guangdong Province, PR China (112° 31′ E 23° 10′ N). Strains DHOA06 and 4 M-K27 grew at pH 4.5–7.0 (optimum, pH 5.0–6.0) and pH 4.5–6.5 (pH 6.0), respectively. Both strains grew at 12–37 °C (optimum, 28 °C) and NaCl levels up to 1.0 % (optimum 0 %, w/v). Phylogenetic analysis based on both 16S rRNA gene sequences and the concatenated partial atpD, gyrB andlepA gene sequences showed that strains DHOA06 and 4 M-K27 formed two isolated clades with members of the genus Dyella, but they each occupied a distinctive position within the genus. Strains DHOA06 and 4 M-K27 showed the highest 16S rRNA gene sequence similarities to Dyellacaseinilytica DHOB09 (98.7 %) and Dyellaacidisoli 4M-Z03 (98.8 %), respectively. DNA–DNA hybridization values of strains DHOA06/DHOB09 and 4 M-K27/4M-Z03 were 27.4±2.4 % and 38.8±1.0 %, respectively. Ubiquinone-8 was the only respiratory quinone detected in both strains. Their major fatty acids consisted of iso-C15 : 0, iso-C16 : 0 and iso-C17 : 1 ω9c, and strain DHOA06 had iso-C17 : 0 in addition. Their polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified aminophospholipid, one unidentified phospholipid and two unidentified aminolipids, and strain DHOA06 had phosphatidylmethylethanolamine and one unidentified lipid in addition. The DNA G+C contents of strains DHOA06 and 4 M-K27 were 59.1 and 61.7 mol%, respectively. Based on the above results, we propose that strains DHOA06 and 4 M-K27 represent two novel species of the genus Dyella , namely Dyelladinghuensis sp. nov. (type strain DHOA06 = KCTC 52129=NBRC 111978) and Dyellachoica sp. nov. (type strain 4 M-K27=GDMCC 1.1189=LMG 30267).

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  • Accepted:
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Keyword(s): choica , dinghuensis , Dyella , phylogeny and taxonomy
© 2019 IUMS
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2019-03-22
2024-04-20
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Dyella dinghuensis sp. nov. and Dyella choica sp. nov., isolated from forest soil
Int J Syst Evol Microbiol 69, 1496 (2019); https://doi.org/10.1099/ijsem.0.003356
/content/journal/ijsem/10.1099/ijsem.0.003356
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