Amphritea opalescens sp. nov., isolated from sediment adjacent to Fildes Peninsula, Antarctica
Han, Xiaoyan and Zhang, Jing and Zhang, Yanfeng and Liu, Juanjuan and Fang, Wei and Fang, Zemin and Xiao, Yazhong,, 69, 1585-1590 (2019),
doi = https://doi.org/10.1099/ijsem.0.003359,
publicationName = Microbiology Society,
issn = 1466-5026,
abstract= A taxonomic study was carried out on a Gram-stain-negative bacterium, namely strain ANRC-JH13T, isolated from a sediment sample collected at Jasper beach, adjacent to Fildes Peninsula, Antarctica. Cells of strain ANRC-JH13T were non-spore-forming rods and motile by the way of flagellum. Strain ANRC-JH13T was facultatively anaerobic, oxidase-positive, and catalase-positive. Growth of strain ANRC-JH13T occurred at 10–42 °C (optimum, 28 °C), pH 4.0–11.0 (pH 7.0) and 0–12.0 % (w/v) NaCl (1.0–2.0 %). Its predominant fatty acids were C16 : 0 (21.7 %), summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c; 38.3 %), and summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c; 20.1 %). Isoprenoid quinone Q-8 was the major respiratory quinone. Its major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two unidentified aminolipids, and four unknown polar lipids. The DNA G+C content was 48 mol%. Strain ANRC-JH13T showed the highest 16S rRNA gene sequence similarity to Amphritea balenae JAMM 1525T (97.9 %), followed by Amphritea atlantica M41T (97.8 %) and Amphritea japonica JAMM 1866T (97.3 %), and formed a lineage within the genus Amphritea on the phylogenetic trees. However, the in silico average nucleotide identity values between strain ANRC-JH13T and A. balenae JAMM 1525T, A. atlantica M41T, and A. japonica JAMM 1866T were 74.0, 76.7, and 74.9 %, respectively. The in silico DNA–DNA hybridization values between them were 19.8, 20.6, and 19.4 %, respectively. Based on the results from phenotypic, chemotaxonomic, and phylogenetic analyses, strain ANRC-JH13T is considered to represent a novel species of the genus Amphritea , for which the name Amphritea opalescens sp. nov. is proposed. The type strain is ANRC-JH13T (=MCCC 1K03512T=KCTC 62532T).,
language=,
type=