Flavobacterium piscinae sp. nov., isolated from a fish pond
Chen, Wen-Ming and Guo, Ya-Ping and Kwon, Soon-Wo and Sheu, Shih-Yi,, 69, 1775-1782 (2019),
doi = https://doi.org/10.1099/ijsem.0.003391,
publicationName = Microbiology Society,
issn = 1466-5026,
abstract= Strain ICH-30T was isolated from a freshwater fish pond in Taiwan. Phylogenetic analyses based on 16S rRNA gene sequences and coding sequences of 92 protein clusters indicated that strain ICH-30T formed a phylogenetic lineage in the genus Flavobacterium . Strain ICH-30T was most closely related to Flavobacteriumlacus NP180T with 97.0 % 16S rRNA gene sequence similarity. Strain ICH-30T showed 70.0–83.1 % average nucleotide identity and 16.5–23.7 % digital DNA–DNA hybridization identity with the type strains of other closely related Flavobacterium species. Cells of strain ICH-30T were Gram-stain-negative, strictly aerobic, non-motile, rod-shaped and formed dark orange colonies. Optimal growth occurred at 20–30 °C, pH 8–9 and 0–0.5 % NaCl. Strain ICH-30T contained iso-C15 : 1 G and iso-C15 : 0 as the predominant fatty acids. The major hydroxyl fatty acids were iso-C17 : 0 3-OH, iso-C16 : 0 3-OH and iso-C15 : 0 3-OH. The polar lipid profile consisted of phosphatidylethanolamine, six uncharacterized aminophospholipids, one uncharacterized phospholipid and two uncharacterized lipids. The major polyamine was homospermidine. The only isoprenoid quinone was MK-6. The DNA G+C content of the genomic DNA was 34.3 mol%. On the basis of the phylogenetic inference and phenotypic data, strain ICH-30T should be classified as a novel species, for which the name Flavobacterium piscinae sp. nov. is proposed. The type strain is ICH-30T (=BCRC 81122T=LMG 30579T=KCTC 62513T).,
language=,
type=