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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 69, Issue 7

Devosia naphthalenivorans sp. nov., isolated from East Pacific Ocean sediment Free

Abstract

A Gram-stain-negative bacterium, designated CM5-1, was isolated from a sediment sample collected from the East Pacific Ocean. 16S rRNA gene sequence analysis revealed that strain CM5-1 belongs to the genus Devosia , with closely related type strains Devosia submarina KMM 9415 (98.6 %), Devosia psychrophila Cr7-05 (98.6 %) and Devosia psychrophila E84 (98.2 %). Up-to-date bacterial core gene set analysis revealed that strain CM5-1 represents one independent lineage with D. submarina KMM 9415. The average nucleotide identity values of CM5-1 with D. submarina KMM 9415 and D. psychrophila Cr7-05 are 80.1 and 77.9 %, respectively. In silico DNA–DNA hybridization values between strain CM5-1 and D. submarina KMM 9415 and D. psychrophila Cr7-05 are 23.8 and 21.9 %, respectively. Strain CM5-1 contains diphosphatidylglycerol, phosphatidylglycerol and glycolipid as major polar lipids. The sole isoprenoid quinone is ubiquinone-10, and C18 : 1ω7c and 11-methyl C18 : 1ω7c are the dominant cellular fatty acids. The G+C content of the genomic DNA is 61.4 mol%. Differential phylogenetic distinctiveness and chemotaxonomic differences, together with the phenotypic properties observed in this study, revealed that strain CM5-1 could be differentiated from closely related species. Therefore, we propose strain CM5-1 as a novel species of the genus Devosia , for which the name Devosia naphthalenivorans sp. nov. is suggested. The type strain is CM5-1 (=JCM32509=CGMCC 1.13553).

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Keyword(s): 16S rRNA gene , average nucleotide identity , Devosia , in silico DNA–DNA hybridization and up-to-date bacterial core gene
© 2019 IUMS
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2019-05-02
2024-04-25
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Devosia naphthalenivorans sp. nov., isolated from East Pacific Ocean sediment
Int J Syst Evol Microbiol 69, 1974 (2019); https://doi.org/10.1099/ijsem.0.003410
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