Rhizobium chutanense sp. nov., isolated from root nodules of Phaseolus vulgaris in China
Huo, Yunyun and Tong, Wenjun and Wang, Juanjuan and Wang, Fang and Bai, Wenqing and Wang, Entao and Shi, Peng and Chen, Weimin and Wei, Gehong,, 69, 2049-2056 (2019),
doi = https://doi.org/10.1099/ijsem.0.003430,
publicationName = Microbiology Society,
issn = 1466-5026,
abstract= Two Gram-stain-negative, rod-shaped bacterial strains (C5T and C16), isolated from root nodules of Phaseolus vulgaris L. in Jiangxi Province, PR China, were characterized by using a polyphasic taxonomical approach. The phylogenetic analysis of the 16S rRNA gene and three concatenated housekeeping genes (recA–glnII–atpD) revealed that C5T and C16 were members of the genus Rhizobium , yet were distinct from known species. The case for strain C5T representing a novel species was supported by genomic results. Pairwise digital DNA–DNA hybridization and average nucleotide identity values were much lower than the proposed and generally accepted species boundaries. The genome-based phylogenetic tree reconstructed by using the up-to-date bacterial core gene set consisting of 92 genes showed that the strains formed a monophyletic branch, further supporting this result. The symbiotic genes of nodC and nifH were identified in both strains; each could nodulate Phaseolus vulgaris and Glycine max but not Leucaena leucocephala, Pisum sativum or Medicago sativa plants. Major cellular fatty acids of C5T were summed feature 8 (C18 : 1 ω7c/C18 : 1 ω6c; 58.8 %), C18 : 1 ω7c 11-methyl (14.2 %) and C18 : 0 (8.1 %). The DNA G+C content of C5T was 61.4 mol%. Based on these genomic, chemotaxonomic and phenotypic characteristics, we propose a novel species: Rhizobium chutanense sp. nov. The type strain is C5T (=CCTCC AB 2018143T=LMG 30777T).,
language=,
type=