@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.003446, author = "Benga, Laurentiu and Spröer, Cathrin and Schumann, Peter and Verbarg, Susanne and Bunk, Boyke and Engelhardt, Eva and Benten, W. Peter M. and Sager, Martin", title = "Leucobacter muris sp. nov., isolated from the nose of a laboratory mouse", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2019", volume = "69", number = "7", pages = "2095-2100", doi = "https://doi.org/10.1099/ijsem.0.003446", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003446", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Leucobacter", keywords = "laboratory mouse", keywords = "taxonomy", abstract = "A Gram-stain-positive, rod-shaped, aerobic, non-motile, white, opaque bacterial isolate, designated 924/12T, was isolated from the nose of a laboratory mouse in Düsseldorf, Germany. The 16S rRNA gene sequence analyses indicated the phylogenetic position of the strain within the genus Leucobacter . Similarity levels over 97 % were recorded between the 16S rRNA gene sequence of strain 924/12T and the type strains of the species Leucobacter chironomi DSM 19883T (99.5 %), followed by Leucobacter celer subsp. astrifaciens CBX151T (97.6 %), Leucobacter celer subsp. celer NAL101T (97.5 %), ‘ Leucobacter kyeonggiensis ’ F3-P9 (97.5 %), Leucobacter zeae CC-MF41T (97.3 %), Leucobacter chromiiresistens JG31T (97.1 %), Leucobacter triazinivorans JW-1T (97.1 %), Leucobacter corticis 2 C-7T (97.0 %) and Leucobacter aridicolis CIP108388T (97.0 %). DNA–DNA hybridization and whole genomic comparison, mandatory to taxonomically separate strain 924/12T from the type strain of L. chironomi , revealed similarity values of 40.4 and 30.8 %, respectively, thus below the threshold of 70 % recommended differentiating between species. The cell-wall amino acids of the novel isolate were diaminobutyric acid, alanine, glycine, threonine and glutamic acid. The major fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, glycolipid and one unknown lipid, whereas the predominant menaquinones were MK-11 and MK-10. The genomic DNA G+C content of strain 924/12T was 70.6 mol%. Phylogenetic analyses based on the 16S rRNA gene sequences and the phenotypical differences between strain 924/12T and the other closely related type strains of the genus Leucobacter indicated that strain 924/12T represents a novel species within the genus Leucobacter , family Microbacteriaceae , for which the name Leucobacter muris sp. nov. is proposed. The type strain is 924/12T (=DSM 101948T=CCM 8761T).", }