@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.003450, author = "Feng, Guang-Da and Zhang, Jun and Zhang, Xian-Jiao and Wang, Sheng-Nan and Xiong, Xiong and Zhang, Yu-Lian and Huang, Hua-Rui and Zhu, Hong-Hui", title = "Hymenobacter metallilatus sp. nov., isolated from abandoned lead–zinc ore", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2019", volume = "69", number = "7", pages = "2142-2146", doi = "https://doi.org/10.1099/ijsem.0.003450", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003450", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "dDDH", keywords = "plasmid", keywords = "Hymenobacter metallilatus", keywords = "whole genome sequence", keywords = "ANI", abstract = "An aerobic, non-motile, Gram-stain-negative, red-to-pinkish and rod-shaped bacterium, designated 9PBR-2T, was isolated from an abandoned lead–zinc ore sample collected from Meizhou, Guangdong Province, PR China. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain 9PBR-2T belongs to the genus Hymenobacte r and was most closely related to Hymenobacter rigui KCTC 12533T (98.0 %), Hymenobacter swuensis KCTC 32018T (97.8 %) and Hymenobacter perfusus LMG 26000T (97.6 %). The calculated average nucleotide identity values based on whole genome sequences between strain 9PBR-2T and closely related type strains ranged from 81.3 to 84.1 %. Correspondingly, the digital DNA-DNA hybridization values ranged from 25.5 to 28.1 %. The major fatty acids of strain 9PBR-2T were iso-C15:0, anteiso-C15:0, C16:1 ω5c, summed feature 3 (C16:1 ω6c and/or C16:1 ω7c) and summed feature 4 (iso-C17:1 I and/or anteiso-C17:1 B). It contained menaquinone 7 (MK-7) as the major isoprenoid quinone and phosphatidylethanolamine as the major polar lipid. The genomic DNA G+C content based on whole genome sequence was 59.8 mol%. Characterization based on phylogenetic, chemotaxonomic and phenotypic analyses clearly indicated that strain 9PBR-2T represents a novel species of the genus Hymenobacter , for which the name Hymenobacter metallilatus sp. nov. is proposed. The type strain is 9PBR-2T (=GDMCC 1.1492T=JCM 32699T).", }