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Volume 69, Issue 8

sp. nov., isolated from ground beef, and sp. nov., isolated from a human foot wound Free

Abstract

Two unusual catalase-negative, Gram-stain-positive, -like isolates that were referred to the CDC Laboratory for identification are described. Strain SS1994 was isolated from ground beef and strain SS1995 was isolated from a human foot wound. Comparative 16S rRNA gene sequence analysis of isolates SS1994 and SS1995 against type strain sequences supported their inclusion in the genus . Strain SS1994 showed high sequence similarity (>97.0 %) to the two most recently proposed species, (99.2 %) and (99.0 %) followed by (98.8 %), strain SS1995 (98.6 %), (98.0 %), (98.0 %) and (97.9 %). The 16S rRNA gene sequence of strain SS1995 was most similar to (99.1 %), followed by SS1994 (98.6 %), (98.4 %), (98.1 %), (97.8 %), and both and (97.5 %). A polyphasic taxonomic study using conventional biochemical and the rapid ID 32 STREP system, MALDI-TOF MS, cell fatty acid analysis, pairwise sequence comparisons of the 16S rRNA, , , and genes, and comparative core and whole genome sequence analyses revealed that strains SS1994 and SS1995 were two novel species. The novel taxonomic status of the two isolates was confirmed with core genome phylogeny, average nucleotide identity <84 % and DNA–DNA hybridization <28 % to any other species. The names SS1994=(CCUG 70831=LMG 30164) and SS1995=(CCUG 70832=LMG 30165) are proposed.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Firmicutes , taxonomy , Vagococcus , Vagococcus bubulae and Vagococcus vulneris
© 2019 IUMS
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2019-08-01
2024-04-20
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Vagococcus bubulae sp. nov., isolated from ground beef, and Vagococcus vulneris sp. nov., isolated from a human foot wound
Int J Syst Evol Microbiol 69, 2268 (2019); https://doi.org/10.1099/ijsem.0.003459
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