RT Journal Article SR Electronic(1) A1 Shewmaker, Patricia L. A1 Whitney, Anne M. A1 Gulvik, Christopher A. A1 Humrighouse, Ben W. A1 Gartin, Jarrett A1 Moura, Hercules A1 Barr, John R. A1 Moore, Edward R. B. A1 Karlsson, Roger A1 Pinto, Tatiana C. A. A1 Teixeira, Lucia M.YR 2019 T1 Vagococcus bubulae sp. nov., isolated from ground beef, and Vagococcus vulneris sp. nov., isolated from a human foot wound JF International Journal of Systematic and Evolutionary Microbiology, VO 69 IS 8 SP 2268 OP 2276 DO https://doi.org/10.1099/ijsem.0.003459 PB Microbiology Society, SN 1466-5034, AB Two unusual catalase-negative, Gram-stain-positive, Vagococcus -like isolates that were referred to the CDC Streptococcus Laboratory for identification are described. Strain SS1994T was isolated from ground beef and strain SS1995T was isolated from a human foot wound. Comparative 16S rRNA gene sequence analysis of isolates SS1994T and SS1995T against Vagococcus type strain sequences supported their inclusion in the genus Vagococcus . Strain SS1994T showed high sequence similarity (>97.0 %) to the two most recently proposed species, Vagococcus martis (99.2 %) and Vagococcus teuberi (99.0 %) followed by Vagococcus penaei (98.8 %), strain SS1995T (98.6 %), Vagococcus carniphilus (98.0 %), Vagococcus acidifermentans (98.0 %) and Vagococcus fluvialis (97.9 %). The 16S rRNA gene sequence of strain SS1995T was most similar to V. penaei (99.1 %), followed by SS1994T (98.6 %), V. martis (98.4 %), V. teuberi (98.1 %), V. acidifermentans (97.8 %), and both V. carniphilus and V. fluvialis (97.5 %). A polyphasic taxonomic study using conventional biochemical and the rapid ID 32 STREP system, MALDI-TOF MS, cell fatty acid analysis, pairwise sequence comparisons of the 16S rRNA, rpoA, rpoB, pheS and groL genes, and comparative core and whole genome sequence analyses revealed that strains SS1994T and SS1995T were two novel Vagococcus species. The novel taxonomic status of the two isolates was confirmed with core genome phylogeny, average nucleotide identity <84 % and in silico DNA–DNA hybridization <28 % to any other Vagococcus species. The names Vagococcus bubulae SS1994T=(CCUG 70831T=LMG 30164T) and Vagococcus vulneris SS1995T=(CCUG 70832T=LMG 30165T) are proposed., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003459